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Literature summary extracted from

  • Stephan, A.; Wermann, M.; Von Bohlen, A.; Koch, B.; Cynis, H.; Demuth, H.; Schilling, S.
    Mammalian glutaminyl cyclases and their isoenzymes have identical enzymatic characteristics (2009), FEBS J., 276, 6522-6536.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.5 modified N-terminal region, His-tagged version, expressed in Pichia pastoris Mus musculus
2.3.2.5 modified N-terminal region, His-tagged version, expressed in Pichia pastoris Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.5 C369A improvement of enzyme secretion (expressed in Pichia pastoris) Homo sapiens
2.3.2.5 I73N artificial glycosylation site Homo sapiens
2.3.2.5 I73N/C369A further improvement of enzyme secretion (expressed in Pichia pastoris) Homo sapiens
2.3.2.5 I74N artificial glycosylation site Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.5 benzimidazole competitive Homo sapiens
2.3.2.5 benzimidazole competitive Mus musculus
2.3.2.5 benzylimidazole competitive Homo sapiens
2.3.2.5 benzylimidazole competitive Mus musculus
2.3.2.5 cysteamine
-
 Mus musculus
2.3.2.5 imidazole
-
 Homo sapiens
2.3.2.5 imidazole
-
 Mus musculus
2.3.2.5 L-glutaminyl-7-amido-4-methylcoumarin substrate inhibition Homo sapiens
2.3.2.5 L-glutaminyl-beta-naphthylamide substrate inhibition Homo sapiens
2.3.2.5 L-glutaminyl-beta-naphthylamide substrate inhibition Mus musculus
2.3.2.5 methylimidazole
-
 Homo sapiens
2.3.2.5 methylimidazole
-
 Mus musculus
2.3.2.5 N,N-dimethylcysteamine
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.5 0.029
-
 QEYF protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.03
-
 L-glutaminyl-7-amido-4-methylcoumarin protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.03
-
 L-glutaminyl-7-amido-4-methylcoumarin protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 0.032
-
 L-glutaminyl-beta-naphthylamide protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.035
-
 L-glutaminyl-beta-naphthylamide protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 0.04
-
 QEYF protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 0.058
-
 Gln-Tyr-Ala protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.06
-
 Gln-Phe-Ala protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.08
-
 Gln-Tyr-Ala protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 0.092
-
 Gln-Gln protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.1
-
 Gln-Phe-Ala protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 0.102
-
 Gln-Gly-Pro protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.11
-
 Gln-Gln protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 0.16
-
 Gln-Gly protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.16
-
 QEDL protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.16
-
 QEDL protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 0.23
-
 Gln-Gly-Pro protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 0.36
-
 Gln-Gly protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 0.47
-
 Gln-Glu protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.61
-
 Gln-Glu protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.5 Golgi complex
-
 Homo sapiens
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.5 Homo sapiens
-
-
-
2.3.2.5 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.5 His-tagged version Homo sapiens
2.3.2.5 hydrophobic interaction chromatography, ion exchange chromatography, gel filtration Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.5 Gln-Gln
-
 Mus musculus pGlu-Gln + NH3
-
 ?
2.3.2.5 Gln-Gln
-
 Homo sapiens pGlu-Gln + NH3
-
 ?
2.3.2.5 Gln-Glu
-
 Mus musculus pGlu-Glu + NH3
-
 ?
2.3.2.5 Gln-Glu
-
 Homo sapiens pGlu-Glu + NH3
-
 ?
2.3.2.5 Gln-Gly
-
 Mus musculus pGlu-Gly + NH3
-
 ?
2.3.2.5 Gln-Gly
-
 Homo sapiens pGlu-Gly + NH3
-
 ?
2.3.2.5 Gln-Gly-Pro
-
 Mus musculus pGlu-Gly-Pro + NH3
-
 ?
2.3.2.5 Gln-Gly-Pro
-
 Homo sapiens pGlu-Gly-Pro + NH3
-
 ?
2.3.2.5 Gln-Phe-Ala
-
 Mus musculus pGlu-Phe-Ala + NH3
-
 ?
2.3.2.5 Gln-Phe-Ala
-
 Homo sapiens pGlu-Phe-Ala + NH3
-
 ?
2.3.2.5 Gln-Tyr-Ala
-
 Mus musculus pGlu-Tyr-Ala + NH3
-
 ?
2.3.2.5 Gln-Tyr-Ala
-
 Homo sapiens pGlu-Tyr-Ala + NH3
-
 ?
2.3.2.5 L-glutaminyl-7-amido-4-methylcoumarin
-
 Mus musculus pGlu-7-amido-4-methylcoumarin + NH3
-
 ?
2.3.2.5 L-glutaminyl-7-amido-4-methylcoumarin
-
 Homo sapiens pGlu-7-amido-4-methylcoumarin + NH3
-
 ?
2.3.2.5 L-glutaminyl-beta-naphthylamide
-
 Mus musculus pGlu-beta-naphthylamide + NH3
-
 ?
2.3.2.5 L-glutaminyl-beta-naphthylamide
-
 Homo sapiens pGlu-beta-naphthylamide + NH3
-
 ?
2.3.2.5 QEDL
-
 Mus musculus pEEDL + NH3
-
 ?
2.3.2.5 QEDL
-
 Homo sapiens pEEDL + NH3
-
 ?
2.3.2.5 QEYF
-
 Mus musculus pEEYF + NH3
-
 ?
2.3.2.5 QEYF
-
 Homo sapiens pEEYF + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.5 glutaminyl cyclase isoenzyme Mus musculus
2.3.2.5 glutaminyl cyclase isoenzyme Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.5 1.07
-
 L-glutaminyl-7-amido-4-methylcoumarin protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 1.65
-
 Gln-Gly protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 2.6
-
 Gln-Glu protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 2.7
-
 Gln-Gln protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 3.3
-
 QEYF protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 3.4
-
 L-glutaminyl-beta-naphthylamide protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 4
-
 Gln-Gly-Pro protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 4.57
-
 Gln-Gly protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 6.4
-
 QEDL protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 6.4
-
 QEDL protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 6.98
-
 L-glutaminyl-7-amido-4-methylcoumarin protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 7.5
-
 Gln-Phe-Ala protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 7.7
-
 Gln-Tyr-Ala protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 7.79
-
 Gln-Glu protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 8.66
-
 Gln-Gln protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 11.4
-
 Gln-Gly-Pro protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 11.78
-
 QEYF protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 17.48
-
 L-glutaminyl-beta-naphthylamide protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 22.9
-
 Gln-Tyr-Ala protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 24.1
-
 Gln-Phe-Ala protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.5 7.5 8 for kcat/KM Mus musculus
2.3.2.5 7.5 8 for kcat/KM Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.2.5 0.0039
-
 benzylimidazole protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.0062
-
 benzylimidazole protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 0.027
-
 N,N-dimethylcysteamine protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.052
-
 methylimidazole protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.069
-
 cysteamine protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.082
-
 methylimidazole protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 0.103
-
 imidazole protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.124
-
 benzimidazole protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 0.235
-
 imidazole protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 0.25
-
 benzimidazole protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 1.57
-
 L-glutaminyl-beta-naphthylamide protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 3.55
-
 L-glutaminyl-beta-naphthylamide protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 4.47
-
 L-glutaminyl-7-amido-4-methylcoumarin protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.2.5 9
-
 Gln-Glu protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 9
-
 Gln-Gly protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 16
-
 Gln-Glu protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 28
-
 Gln-Gly protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 38
-
 Gln-Gly-Pro protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 54
-
 Gln-Gln protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 55
-
 QEDL protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 95
-
 Gln-Gln protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 103
-
 L-glutaminyl-7-amido-4-methylcoumarin protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 104
-
 QEDL protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 111
-
 Gln-Gly-Pro protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 117
-
 Gln-Phe-Ala protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 123
-
 QEYF protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 207
-
 Gln-Tyr-Ala protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 229
-
 L-glutaminyl-beta-naphthylamide protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.3.2.5 311
-
 L-glutaminyl-7-amido-4-methylcoumarin protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 394
-
 Gln-Tyr-Ala protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 403
-
 Gln-Phe-Ala protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 413
-
 QEYF protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
2.3.2.5 554
-
 L-glutaminyl-beta-naphthylamide protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus