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Literature summary extracted from

  • Posner, M.G.; Upadhyay, A.; Bagby, S.; Hough, D.W.; Danson, M.J.
    A unique lipoylation system in the Archaea. Lipoylation in Thermoplasma acidophilum requires two proteins (2009), FEBS J., 276, 4012-4022.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.1.20 ATP assay in the presence of Thermoplasma acidophilum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.20 expressed in Escherichia coli as a His-tagged fusion protein Thermoplasma acidophilum

Protein Variants

EC Number Protein Variants Comment Organism
6.3.1.20 additional information LplA and CTD encoding genes are expressed as fusion proteins in Escherichia coli by omitting the stop codon of lplA and the start codon of ctd. Fusion protein is shown to be catalytically active Thermoplasma acidophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.20 MgCl2 assay in the presence of Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.20 11000
-
 SDS-PAGE, molecular weight of His–CTD fusion protein Thermoplasma acidophilum
6.3.1.20 32000
-
 SDS-PAGE, molecular weight of His–LplA fusion protein Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.20 additional information Thermoplasma acidophilum it is shown that E2 lipoyl domain peptide, LplA and CTD are physically associated. LplA and CTD exist in a ratio 1:1 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.20 Thermoplasma acidophilum Q9HKT1 lipoylation occurs in vivo in Thermoplasma acidophilum but requires two proteins LplA and CTD
-
6.3.1.20 Thermoplasma acidophilum Q9HKT2 lipoylation occurs in vivo in Thermoplasma acidophilum but requires two proteins LplA and CTD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.20 using His-tag affinity purification Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.20 additional information it is shown that E2 lipoyl domain peptide, LplA and CTD are physically associated. LplA and CTD exist in a ratio 1:1 Thermoplasma acidophilum ?
-
 ?
6.3.1.20 additional information when the Thermoplasma acidophilum E2 lipoyl domain peptide is incubated with either His–LplA or His–CTD in the presence of ATP, MgCl2 and lipoic acid, no lipoylation is detected. Incubation with both His–LplA and His–CTD in the one reaction results in the lipoylation of the E2 lipoyl domain peptide as indicated by nondenaturing PAGE Thermoplasma acidophilum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.20 CTD
-
 Thermoplasma acidophilum
6.3.1.20 LPLA
-
 Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.20 25
-
 assay at Thermoplasma acidophilum