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Literature summary extracted from

  • Igloi, G.L.; Schiefermayr, E.
    Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants (2009), FEBS J., 276, 1307-1318.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.19 into the vector pET32a for expression in Escherichia coli BL21 cells Canavalia ensiformis
6.1.1.19 into the vector pET32a for expression in Escherichia coli BL21 cells Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.19 0.0022
-
 L-arginine assay method, aminoacylation of transcript tRNA with [14C]-labelled amino acid Glycine max
6.1.1.19 0.003
-
 L-arginine assay method, aminoacylation of [32P]-labelled transcript tRNA Glycine max
6.1.1.19 0.0078
-
 L-arginine assay method, aminoacylation of [32P]-labelled transcript tRNA Canavalia ensiformis
6.1.1.19 0.0196
-
 L-arginine assay method, aminoacylation of transcript tRNA with [14C]-labelled amino acid Canavalia ensiformis
6.1.1.19 0.0453
-
 L-canavanine assay method, aminoacylation of transcript tRNA with [14C]-labelled amino acid Glycine max
6.1.1.19 0.0454
-
 L-canavanine assay method, aminoacylation of [32P]-labelled transcript tRNA Glycine max
6.1.1.19 1.32
-
 L-canavanine assay method, aminoacylation of [32P]-labelled transcript tRNA Canavalia ensiformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.19 67400
-
 theoretical Canavalia ensiformis
6.1.1.19 68200
-
 theoretical Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.19 ATP + L-arginine + tRNAArg Canavalia ensiformis
-
 AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
6.1.1.19 ATP + L-arginine + tRNAArg Glycine max
-
 AMP + diphosphate + L-arginyl-tRNAArg
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.19 Canavalia ensiformis B2G3G6
-
-
6.1.1.19 Glycine max B6ETP1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.19 utilizing the His-tag, the tag is removed by thrombin treatment Canavalia ensiformis
6.1.1.19 utilizing the His-tag, the tag is removed by thrombin treatment, furthermore a Source15Q column is used Glycine max

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.19 additional information
-
 L-arginine, aminoacylation 100% Canavalia ensiformis
6.1.1.19 additional information
-
 L-arginine, aminoacylation 100% Glycine max
6.1.1.19 additional information
-
 L-canavanine, aminoacylation 15% Canavalia ensiformis
6.1.1.19 additional information
-
 L-canavanine, aminoacylation 45% Glycine max
6.1.1.19 additional information
-
 L-thioarginine, aminoacylation 82% Glycine max
6.1.1.19 additional information
-
 L-thioarginine, aminoacylation 90% Canavalia ensiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.19 ATP + L-arginine + tRNAArg
-
 Canavalia ensiformis AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
6.1.1.19 ATP + L-arginine + tRNAArg
-
 Glycine max AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
6.1.1.19 ATP + L-canavanine + tRNAArg
-
 Canavalia ensiformis AMP + diphosphate + L-canavanyl-tRNAArg
-
 ?
6.1.1.19 ATP + L-canavanine + tRNAArg
-
 Glycine max AMP + diphosphate + L-canavanyl-tRNAArg
-
 ?
6.1.1.19 ATP + L-thioarginine + tRNAArg
-
 Canavalia ensiformis AMP + diphosphate + L-thioarginyl-tRNAArg
-
 ?
6.1.1.19 ATP + L-thioarginine + tRNAArg
-
 Glycine max AMP + diphosphate + L-thioarginyl-tRNAArg
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.19 Arginyl-tRNA synthetase
-
 Canavalia ensiformis
6.1.1.19 Arginyl-tRNA synthetase
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.19 30
-
 aminoacylation assay Canavalia ensiformis
6.1.1.19 30
-
 aminoacylation assay Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.19 7.5
-
 aminoacylation assay Canavalia ensiformis
6.1.1.19 7.5
-
 aminoacylation assay Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.19 ATP
-
 Canavalia ensiformis
6.1.1.19 ATP
-
 Glycine max