Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Quinn, J.M.; Tam, S.; Sims, N.A.; Saleh, H.; McGregor, N.E.; Poulton, I.J.; Scott, J.W.; Gillespie, M.T.; Kemp, B.E.; van Denderen, B.J.
    Germline deletion of AMP-activated protein kinase beta subunits reduces bone mass without altering osteoclast differentiation or function (2010), FASEB J., 24, 275-285.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.31 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside activates AMPK in BMMs and RAW264.7 cells. While 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside greatly stimulates osteoclast formation, it acts through an AMPK-independent mechanism Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.31 A134974 at 1 nM ablates the stimulatory action of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside with no effects on osteoclast formation in the absence of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside Mus musculus
2.7.11.31 additional information osteoclasts and macrophages generated from AMPK beta1-/- mice display no detectable AMPK activity Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.31 Mus musculus
-
 C57BL/6J mice
-
2.7.11.31 Mus musculus C57/BL6J
-
 C57BL/6J mice
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.31 bone
-
 Mus musculus
-
2.7.11.31 additional information present in BMM cells. Osteoclasts and macrophages lack AMPK beta2 subunit expression Mus musculus
-
2.7.11.31 osteoblast
-
 Mus musculus
-
2.7.11.31 RAW-264.7 cell
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.31 ATP + acetyl-CoA carboxylase
-
 Mus musculus ADP + [acetyl-CoA carboxylase]phosphate
-
 ?
2.7.11.31 ATP + acetyl-CoA carboxylase
-
 Mus musculus C57/BL6J ADP + [acetyl-CoA carboxylase]phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.31 heterotrimer immunoprecipitation Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.31 AMP-activated protein kinase
-
 Mus musculus
2.7.11.31 AMPK
-
 Mus musculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.11.31 0.048
-
-
 Mus musculus A134974

Expression

EC Number Organism Comment Expression
2.7.11.31 Mus musculus loss of AMPK beta-subunit expression in beta1-/- and beta2-/- mice, leading to a low-bone-mass phenotype down

General Information

EC Number General Information Comment Organism
2.7.11.31 malfunction germline deletion of either AMPK beta1 or beta2 subunit isoforms results in reduced trabecular bone density and mass, but without effects on osteoclast or osteoblast numbers, as compared to wild-type littermate controls Mus musculus
2.7.11.31 physiological function AMPK is required to maintain normal bone density, but not through bone cell differentiation, and does not mediate powerful osteolytic effects of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside Mus musculus