EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.99.3 | expressed in Escherichia coli Codon Plus BL21[pREP4] cells | Arabidopsis thaliana |
4.1.99.3 | GST-tagged class II CPD photolyase overexpressed in Escherichia coli | Oryza sativa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.99.3 | E109A | is catalytically active but unable to bind the second cofactor methenyltetrahydrofolate | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.99.3 | Arabidopsis thaliana | - |
- |
- |
4.1.99.3 | Escherichia coli | - |
- |
- |
4.1.99.3 | Oryza sativa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.99.3 | - |
Arabidopsis thaliana |
4.1.99.3 | - |
Oryza sativa |
EC Number | Storage Stability | Organism |
---|---|---|
4.1.99.3 | -70°C, 0.05 M sodium phosphate, pH 7.0, 0.1 M NaCl, 20% (v/v) glycerol | Escherichia coli |
4.1.99.3 | -70°C, 0.05 M sodium phosphate, pH 7.0, 0.1 M NaCl, 20% (v/v) glycerol | Arabidopsis thaliana |
4.1.99.3 | -70°C, 0.05 M sodium phosphate, pH 7.0, 0.1 M NaCl, 20% (v/v) glycerol | Oryza sativa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.99.3 | class I CPD photolyase | - |
Escherichia coli |
4.1.99.3 | class I cyclobutane pyrimidine dimer photolyase | - |
Escherichia coli |
4.1.99.3 | class I photolyase | - |
Escherichia coli |
4.1.99.3 | class II CPD photolyase | - |
Arabidopsis thaliana |
4.1.99.3 | class II CPD photolyase | - |
Oryza sativa |
4.1.99.3 | class II cyclobutane pyrimidine dimer photolyase | - |
Arabidopsis thaliana |
4.1.99.3 | class II cyclobutane pyrimidine dimer photolyase | - |
Oryza sativa |
4.1.99.3 | class II photolyase | - |
Arabidopsis thaliana |
4.1.99.3 | class II photolyase | - |
Oryza sativa |
4.1.99.3 | CPD photolyase | - |
Escherichia coli |
4.1.99.3 | CPD photolyase | - |
Arabidopsis thaliana |
4.1.99.3 | CPD photolyase | - |
Oryza sativa |
4.1.99.3 | CPDI | - |
Escherichia coli |
4.1.99.3 | CPDII | - |
Arabidopsis thaliana |
4.1.99.3 | CPDII | - |
Oryza sativa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.99.3 | FAD | - |
Escherichia coli | |
4.1.99.3 | FAD | binds the flavin cofactor in a pocket that is conserved in terms of its electronic properties | Arabidopsis thaliana | |
4.1.99.3 | FAD | binds the flavin cofactor in a pocket that is conserved in terms of its electronic properties. W399-W378-W406 may function as potential electron donors to the flavin and are possible candidate tryptophans for light-induced electron transfer | Oryza sativa | |
4.1.99.3 | FADH2 | photoreduction of FADH proceeds along the conserved tryptophan triad W306-W359-W382 | Escherichia coli | |
4.1.99.3 | methenyltetrahydrofolate | - |
Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.99.3 | metabolism | photoreduction kinetics of class II photolyases are very similar to those of the other classes with even higher photoreduction rates in class II as compared to class I. W399-W378-W406 electron-transfer pathway is conserved among class II CPD photolyase enzymes | Arabidopsis thaliana |
4.1.99.3 | metabolism | photoreduction kinetics of class II photolyases are very similar to those of the other classes with even higher photoreduction rates in class II as compared to class I. W399-W378-W406 electron-transfer pathway is conserved among class II CPD photolyase enzymes | Oryza sativa |