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Literature summary extracted from

  • Ritschel, T.; Hoertner, S.; Heine, A.; Diederich, F.; Klebe, G.
    Crystal structure analysis and in silico pKa calculations suggest strong pKa shifts of ligands as driving force for high-affinity binding to TGT (2009), ChemBioChem, 10, 716-727.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.29 expressed in Escherichia coli Zymomonas mobilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.29 2-aminolin-benzoguanine inhibitors in complex with TGT, by hanging-drop, vapor diffusion method at 0°C and macro-seeding, to 1.28-1.78 A resolution, crystals belong to space group C2. The 2-amino-lin-benzoguanines are protonated upon binding to TGT. At pH 5.5, Asp102 is rotated to 75% into the guanine binding pocket whereas at pH 8.5 the same residue is oriented to 100% out of the pocket. Pronounced disorder of the attached side chains addressing the ribose 33 binding pocket Zymomonas mobilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.29 2,6-diamino-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 2-amino-7-(dimethylamino)quinazolin-4(3H)-one
-
 Zymomonas mobilis
2.4.2.29 2-amino-7-[benzyl(methyl)amino]quinazolin-4(3H)-one
-
 Zymomonas mobilis
2.4.2.29 6-amino-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 6-amino-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 6-amino-2-methyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 6-amino-2-[(2-morpholin-4-ylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 6-amino-2-[(2-phenylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 6-amino-2-[(naphthalen-1-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 6-amino-2-[(thiophen-2-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.29 Zymomonas mobilis P28720
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.29
-
 Zymomonas mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.29 [tRNATyr]7-aminomethyl-7-carbaguanine + guanine
-
 Zymomonas mobilis [tRNATyr]-guanine + 7-aminomethyl-7-carbaguanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.29 TGT
-
 Zymomonas mobilis
2.4.2.29 tRNA-guanine transglycosylase
-
 Zymomonas mobilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.29 0.000006
-
 6-amino-2-[(2-morpholin-4-ylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 0.00001
-
 6-amino-2-[(2-phenylethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 0.000035
-
 6-amino-2-[(thiophen-2-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 0.000055
-
 6-amino-2-[(naphthalen-1-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 0.000058
-
 6-amino-2-(methylamino)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 0.000077
-
 2,6-diamino-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 0.00076
-
 6-amino-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 0.0015
-
 6-amino-2-methyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 0.0031
-
 2-amino-7-(dimethylamino)quinazolin-4(3H)-one
-
 Zymomonas mobilis
2.4.2.29 0.0041
-
 2-amino-7-[benzyl(methyl)amino]quinazolin-4(3H)-one
-
 Zymomonas mobilis