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Literature summary extracted from

  • Goedl, C.; Nidetzky, B.
    Sucrose phosphorylase harbouring a redesigned, glycosyltransferase-like active site exhibits retaining glucosyl transfer in the absence of a covalent intermediate (2009), ChemBioChem, 10, 2333-2337.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.7 D196N/E237Q the mutation affects the the stereoselectivity of the reaction Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.7 additional information
-
 additional information steady-state kinetic analysis, ping-pong kinetics Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.7 alpha-D-glucose 1-fluoride + phosphate Leuconostoc mesenteroides
-
 fluoride + alpha-D-glucose 1-phosphate
-
 r
2.4.1.7 sucrose + phosphate Leuconostoc mesenteroides
-
 D-fructose + alpha-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.7 Leuconostoc mesenteroides
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.7 alpha-D-glucose 1-fluoride + phosphate
-
 Leuconostoc mesenteroides fluoride + alpha-D-glucose 1-phosphate
-
 r
2.4.1.7 alpha-D-glucose 1-fluoride + phosphate mechanisms for wild-type sucrose phosphorylase and doubly mutated variants, overview Leuconostoc mesenteroides fluoride + alpha-D-glucose 1-phosphate
-
 r
2.4.1.7 alpha-D-glucose 1-phosphate + arsenate
-
 Leuconostoc mesenteroides alpha-D-glucose 1-arsenate + phosphate
-
 ?
2.4.1.7 additional information alpha-retaining glucosyl transfer through front-side bimolecular nucleophilic substitution Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 sucrose + phosphate
-
 Leuconostoc mesenteroides D-fructose + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.7 More model of a ternary complex between mutated sucrose phosphorylase, alpha-gklucosyl 1-fluorid, and phosphate, overview Leuconostoc mesenteroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.7 0.0001
-
 alpha-D-glucose 1-phosphate arsenolysis of alpha-D-glucose 1-phosphate, mutant D196N/E237Q Leuconostoc mesenteroides
2.4.1.7 170
-
 alpha-D-glucose 1-phosphate arsenolysis of alpha-D-glucose 1-phosphate, wild-type enzyme Leuconostoc mesenteroides