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Literature summary extracted from

  • Kita, Y.; Hanafy, M.; Deguchi, M.; Hasegawa, H.; Terakawa, T.; Kitamura, K.; Ishimoto, M.
    Generation and characterization of herbicide-resistant soybean plants expressing novel phosphinothricin N-acetyltransferase genes (2009), Breed. Sci., 59, 245-251.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.3.1.183 agriculture creation of herbicide resistant plants Nocardia sp.
2.3.1.183 agriculture creation of herbicide resistant plants Streptomyces sp.
2.3.1.183 biotechnology creation of herbicide resistant plants Nocardia sp.
2.3.1.183 biotechnology creation of herbicide resistant plants Streptomyces sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.183 expressed in soybean after transformation with Agrobacterium tumefaciens Nocardia sp.
2.3.1.183 expressed in soybean after transformation with Agrobacterium tumefaciens Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.183 21000
-
 SDS-PAGE Nocardia sp.
2.3.1.183 21000
-
 SDS-PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.183 Nocardia sp.
-
 soil isolate
-
2.3.1.183 Streptomyces sp.
-
 soil isolate, DDBJ no. DD355820
-
2.3.1.183 Streptomyces sp. AB3534
-
 soil isolate, DDBJ no. DD355820
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.183
-
 Nocardia sp.
2.3.1.183
-
 Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.183 0.0177
-
 at 30°C, enzyme activity in crude extract of the transgenic soybean line h-7, in wild-type plants no activity is detectable Streptomyces sp.
2.3.1.183 0.0221
-
 at 30°C, enzyme activity in crude extract of the transgenic soybean line m-12, in wild-type plants no activity is detectable Nocardia sp.
2.3.1.183 0.0357
-
 at 30°C, enzyme activity in crude extract of the transgenic soybean line h-9, in wild-type plants no activity is detectable Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.183 phosphinothricin + acetyl-CoA
-
 Nocardia sp. L-N-acetylphosphinothricin + CoA
-
 ?
2.3.1.183 phosphinothricin + acetyl-CoA
-
 Streptomyces sp. L-N-acetylphosphinothricin + CoA
-
 ?
2.3.1.183 phosphinothricin + acetyl-CoA
-
 Streptomyces sp. AB3534 L-N-acetylphosphinothricin + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.183 hpat
-
 Streptomyces sp.
2.3.1.183 MAT
-
 Nocardia sp.
2.3.1.183 methionine sulfone N-acetyltransferase
-
 Nocardia sp.
2.3.1.183 phosphinothricin-N-acetyltransferase
-
 Nocardia sp.
2.3.1.183 phosphinothricin-N-acetyltransferase
-
 Streptomyces sp.