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Literature summary extracted from

  • Kim, H.J.; Park, A.R.; Lee, J.K.; Oh, D.K.
    Characterization of an acid-labile, thermostable beta-glycosidase from Thermoplasma acidophilum (2009), Biotechnol. Lett., 31, 1457-1462.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 additional information not inhibited by EDTA Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.2
-
 4-nitrophenyl-beta-D-fucopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 0.4
-
 4-nitrophenyl-beta-D-glucopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 1.3
-
 4-nitrophenyl-beta-D-mannopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 1.5
-
 4-nitrophenyl-beta-D-galactopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 additional information the enzyme is not activated by the presence of monovalent or divalent cations Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 57000
-
 2 * 57000, SDS-PAGE Thermoplasma acidophilum
3.2.1.21 57070
-
 subunit, MALDI-TOF mass spectrometry Thermoplasma acidophilum
3.2.1.21 114000
-
 native enzyme, gel filtration Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Thermoplasma acidophilum Q9HIL6 strain DSM 1728
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 Hi-Trap column chromatography, gel filtration Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 0.7
-
 after 68fold purification, using 2-nitrophenyl-beta-D-galactopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 1.2
-
 crude extract, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 1.7
-
 after 68fold purification, using 2-nitrophenyl-beta-D-fucopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 1.8
-
 after 68fold purification, using 2-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 4.9
-
 after 68fold purification, using 4-nitrophenyl-beta-D-mannopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 8.5
-
 after 68fold purification, using 4-nitrophenyl-beta-D-galactopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 82
-
 after 68fold purification, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 113
-
 after 68fold purification, using 4-nitrophenyl-beta-D-fucopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Thermoplasma acidophilum 4-nitrophenol + beta-D-fucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactoside + H2O
-
 Thermoplasma acidophilum 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermoplasma acidophilum 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Thermoplasma acidophilum 4-nitrophenol + beta-D-mannopyranose
-
 ?
3.2.1.21 additional information no activity is detected for 4-nitrophenyl-beta-D-xylopyranoside, 4-nitrophenyl-beta-L-arabinopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, and 4-nitrophenyl-alpha-L-arabinopyranoside Thermoplasma acidophilum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 homodimer 2 * 57000, SDS-PAGE Thermoplasma acidophilum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 BglA
-
 Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 90
-
-
 Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 14
-
 4-nitrophenyl-beta-D-mannopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 27
-
 4-nitrophenyl-beta-D-galactopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 141
-
 4-nitrophenyl-beta-D-glucopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 182
-
 4-nitrophenyl-beta-D-fucopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6
-
 the maximum activity of BglA at 75°C is initially at pH 4.5 but after 1 h at 75°C is at pH 6.0 Thermoplasma acidophilum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 4.5 6 the enzyme is unstable below pH 6.0, at pH 6.0 its half-life at 75°C is 28 days but at pH 4.5 is 13 h Thermoplasma acidophilum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.21 11
-
 4-nitrophenyl-beta-D-mannopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 18
-
 4-nitrophenyl-beta-D-galactopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 381
-
 4-nitrophenyl-beta-D-glucopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
3.2.1.21 1068
-
 4-nitrophenyl-beta-D-fucopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum