Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chauve, M.; Mathis, H.; Huc, D.; Casanave, D.; Monot, F.; Lopes Ferreira, N.
    Comparative kinetic analysis of two fungal beta-glucosidases (2010), Biotechnol. Biofuels, 3, 3-3.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 D-glucose competitive inhibition Aspergillus niger
3.2.1.21 D-glucose competitive inhibition Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.38
-
 4-nitrophenyl-beta-D-glucopyranoside in 50 mM citrate buffer, pH 4.8, at 50°C Trichoderma reesei
3.2.1.21 0.57
-
 4-nitrophenyl-beta-D-glucopyranoside in 50 mM citrate buffer, pH 4.8, at 50°C Aspergillus niger
3.2.1.21 0.88
-
 cellobiose in 50 mM citrate buffer, pH 4.8, at 50°C Aspergillus niger
3.2.1.21 1.36
-
 cellobiose in 50 mM citrate buffer, pH 4.8, at 50°C Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Aspergillus niger
-
-
-
3.2.1.21 Trichoderma reesei
-
-
-
3.2.1.21 Trichoderma reesei CL847
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 Mono Q column chromatography Trichoderma reesei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 additional information
-
 the enzyme has a specific activity of 1096438 unots/mg after 53fold purification, at 50°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus niger 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Trichoderma reesei 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Trichoderma reesei CL847 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Aspergillus niger 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Trichoderma reesei 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Trichoderma reesei CL847 2 beta-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 BGL1
-
 Trichoderma reesei
3.2.1.21 SP188 commercial preparation Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 26.37
-
 4-nitrophenyl-beta-D-glucopyranoside in 50 mM citrate buffer, pH 4.8, at 50°C Aspergillus niger
3.2.1.21 31.62
-
 cellobiose in 50 mM citrate buffer, pH 4.8, at 50°C Aspergillus niger
3.2.1.21 40.75
-
 cellobiose in 50 mM citrate buffer, pH 4.8, at 50°C Trichoderma reesei
3.2.1.21 87.93
-
 4-nitrophenyl-beta-D-glucopyranoside in 50 mM citrate buffer, pH 4.8, at 50°C Trichoderma reesei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 2.7
-
 D-glucose using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 50 mM citrate buffer, pH 4.8, at 50°C Aspergillus niger
3.2.1.21 3.25
-
 D-glucose using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 50 mM citrate buffer, pH 4.8, at 50°C Trichoderma reesei
3.2.1.21 3.4
-
 D-glucose using cellobiose as substrate, in 50 mM citrate buffer, pH 4.8, at 50°C Aspergillus niger