Literature summary extracted from
Ryan, J.; Clark, D.
P450cam biocatalysis in surfactant-stabilized two-phase emulsions (2008), Biotechnol. Bioeng., 99, 1311-1319.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.14.15.1 |
bis(2-ethylhexyl) sulfosuccinate |
improves the initial activity of P450cam in two-phase emulsions with initial camphor concentrations of 5-15 mM. P450cam is activated in the surfactant-free emulsions, and addition of bis(2-ethylhexyl) sulfosuccinate sodium salt improves the activity even further, at least over the range of camphor concentrations for which initial rates are readily measurable in all media. The largest rate enhancement is 4.5fold. Nearly 50times more product is formed in the surfactant-stabilized emulsions than is achieved in aqueous buffer |
Pseudomonas putida |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.15.1 |
plasmids transformed into Escherichia coli Bl21(DE3) competent cells |
Pseudomonas putida |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.14.15.1 |
additional information |
emulsions formed by strongly agitating the mixture with a vortexer denatured protein, P450cam has a complete loss of activity when incubated under these conditions for more than 5 min prior to the initiation of the reaction |
Pseudomonas putida |
|
Organic Solvent Stability
EC Number |
Organic Solvent |
Comment |
Organism |
---|
1.14.15.1 |
hexane |
P450cam is stable and retains 80% of its initial activity after 1 h in a hexane/water emulsion at agitation speeds of less than 250 rpm |
Pseudomonas putida |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.15.1 |
Pseudomonas putida |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.15.1 |
by anion exchange, hydrophobic interaction and gel filtration |
Pseudomonas putida |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
1.14.15.1 |
-80°C |
Pseudomonas putida |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.15.1 |
(+)-camphor + O2 + reduced putidaredoxin |
- |
Pseudomonas putida |
exo-5-hydroxycamphor + oxidized putidaredoxin + H2O |
- |
? |
|
1.14.15.1 |
(R)-exo-5-hydroxycamphor + O2 + reduced putidaredoxin |
- |
Pseudomonas putida |
2,5-diketocamphane + oxidized putidaredoxin + H2O |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.15.1 |
P450cam |
- |
Pseudomonas putida |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.15.1 |
cytochrome m |
optimal ratio of putidaredoxin reductase, putidaredoxin, cytochrome m is 1:10:2 |
Pseudomonas putida |
|
1.14.15.1 |
putidaredoxin |
optimal ratio of putidaredoxin reductase, putidaredoxin, cytochrome m is 1:10:2 |
Pseudomonas putida |
|