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Literature summary extracted from

  • Mueller, M.; Takemasa, R.; Schwarz, A.; Atomi, H.; Nidetzky, B.
    "Short-chain" alpha-1,4-glucan phosphorylase having a truncated N-terminal domain: functional expression and characterization of the enzyme from Sulfolobus solfataricus (2009), Biochim. Biophys. Acta, 1794, 1709-1714.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.1 heterologous gene expression of enzyme harboring Strep-Tag II fused to the C-terminal Tyr-465 of the enzyme, in Thermococcus kodakaraensis Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 4
-
phosphate 70°C, pH 7.0 Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 55000
-
x * 55000, SDS-PAGE Saccharolobus solfataricus
2.4.1.1 69000
-
gel filtration, recombinant protein Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Saccharolobus solfataricus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 2
-
similar specific activity for substrates maltodextrin, maltopentaose and starch, 70°C, pH 7.0 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 (maltodextrin)n + phosphate
-
Saccharolobus solfataricus (maltodextrin)n-1 + alpha-D-glucose 1-phosphate
-
?
2.4.1.1 maltopentaose + phosphate
-
Saccharolobus solfataricus maltotetraose + alpha-D-glucose 1-phosphate
-
?
2.4.1.1 maltotetraose + phosphate 50% of the activity with maltopentaose Saccharolobus solfataricus maltotriose + alpha-D-glucose 1-phosphate
-
?
2.4.1.1 additional information no substrate in direction of glucose 1-phosphate synthesis: pullulan, maltotriose Saccharolobus solfataricus ?
-
?
2.4.1.1 starch + alpha-D-glucose 1-phosphate
-
Saccharolobus solfataricus starch + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.1 ? x * 55000, SDS-PAGE Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.1 2.1
-
phosphate 70°C, pH 7.0 Saccharolobus solfataricus