EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.1 | heterologous gene expression of enzyme harboring Strep-Tag II fused to the C-terminal Tyr-465 of the enzyme, in Thermococcus kodakaraensis | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.1 | 4 | - |
phosphate | 70°C, pH 7.0 | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 55000 | - |
x * 55000, SDS-PAGE | Saccharolobus solfataricus |
2.4.1.1 | 69000 | - |
gel filtration, recombinant protein | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.1 | Saccharolobus solfataricus | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 2 | - |
similar specific activity for substrates maltodextrin, maltopentaose and starch, 70°C, pH 7.0 | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.1 | (maltodextrin)n + phosphate | - |
Saccharolobus solfataricus | (maltodextrin)n-1 + alpha-D-glucose 1-phosphate | - |
? | |
2.4.1.1 | maltopentaose + phosphate | - |
Saccharolobus solfataricus | maltotetraose + alpha-D-glucose 1-phosphate | - |
? | |
2.4.1.1 | maltotetraose + phosphate | 50% of the activity with maltopentaose | Saccharolobus solfataricus | maltotriose + alpha-D-glucose 1-phosphate | - |
? | |
2.4.1.1 | additional information | no substrate in direction of glucose 1-phosphate synthesis: pullulan, maltotriose | Saccharolobus solfataricus | ? | - |
? | |
2.4.1.1 | starch + alpha-D-glucose 1-phosphate | - |
Saccharolobus solfataricus | starch + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.1 | ? | x * 55000, SDS-PAGE | Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.1 | 2.1 | - |
phosphate | 70°C, pH 7.0 | Saccharolobus solfataricus |