EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.5.1 | expression in Escherichia coli, with SUMO tag | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.5.1 | 0.021 | - |
deamido-NAD+ | pH 8.3, 37°C | Thermotoga maritima | |
6.3.5.1 | 0.095 | - |
ATP | pH 8.3, 37°C | Thermotoga maritima | |
6.3.5.1 | 0.33 | - |
NH3 | pH 8.3, 37°C | Thermotoga maritima | |
6.3.5.1 | 0.42 | - |
L-Gln | pH 8.3, 37°C | Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.5.1 | Thermotoga maritima | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.5.1 | recombinant enzyme | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.5.1 | ATP + deamido-NAD+ + L-Gln | - |
Thermotoga maritima | AMP + diphosphate + NAD+ + L-Glu | - |
? | |
6.3.5.1 | ATP + deamido-NAD+ + NH3 + H2O | - |
Thermotoga maritima | AMP + diphosphate + NAD+ | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.5.1 | 80 | - |
at temperatures above 65°C the glutamine-dependent reaction becomes about 20% faster than the ammonia-dependent at 80°C | Thermotoga maritima |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.5.1 | 65 | 80 | at temperatures above 65°C the glutamine-dependent reaction becomes about 20% faster than the ammonia-dependent at 80°C | Thermotoga maritima |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.5.1 | 0.15 | - |
ATP | pH 8.3, 37°C | Thermotoga maritima | |
6.3.5.1 | 0.16 | - |
deamido-NAD+ | pH 8.3, 37°C | Thermotoga maritima | |
6.3.5.1 | 0.24 | - |
NH3 | pH 8.3, 37°C | Thermotoga maritima | |
6.3.5.1 | 0.288 | - |
L-Gln | pH 8.3, 37°C | Thermotoga maritima |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.5.1 | 0.68 | - |
L-Gln | pH 8.3, 37°C | Thermotoga maritima | |
6.3.5.1 | 0.7 | - |
NH3 | pH 8.3, 37°C | Thermotoga maritima | |
6.3.5.1 | 1.6 | - |
ATP | pH 8.3, 37°C | Thermotoga maritima | |
6.3.5.1 | 7.6 | - |
deamido-NAD+ | pH 8.3, 37°C | Thermotoga maritima |