EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.6 | construction of a 3D-homology model. This model predicts preservation of the chain topology and three-domain organization typical of this family, locates the least stable structural elements at the flat contact surface between monomers, and shows that alterations in all three domains are required for dimer dissociation | Methanocaldococcus jannaschii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.6 | W387F | kinetic data | Methanocaldococcus jannaschii |
2.5.1.6 | W387F/Y120W | kinetic data | Methanocaldococcus jannaschii |
2.5.1.6 | W387F/Y170W | kinetic data | Methanocaldococcus jannaschii |
2.5.1.6 | W387F/Y226W | kinetic data | Methanocaldococcus jannaschii |
2.5.1.6 | W387F/Y233W | 4.9fold increase in kcat value. Mutant shows three transitions in urea titration curve, contrary to two transitions of wild-type | Methanocaldococcus jannaschii |
2.5.1.6 | W387F/Y255W | fluorescence intensity reduced to 18% of wild-type | Methanocaldococcus jannaschii |
2.5.1.6 | W387F/Y267W | kinetic data | Methanocaldococcus jannaschii |
2.5.1.6 | W387F/Y273W | kinetic data | Methanocaldococcus jannaschii |
2.5.1.6 | W387F/Y323W | kinetic data | Methanocaldococcus jannaschii |
2.5.1.6 | W387F/Y344W | kinetic data | Methanocaldococcus jannaschii |
2.5.1.6 | W387F/Y371W | shows one transition in urea titration curve, contrary to two transitions of wild-type | Methanocaldococcus jannaschii |
2.5.1.6 | W387F/Y49W | decrease in kcat value by 32% | Methanocaldococcus jannaschii |
2.5.1.6 | W387F/Y72W | kinetic data | Methanocaldococcus jannaschii |
2.5.1.6 | W387F/Y85W | kinetic data | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.6 | 0.1 | - |
ATP | mutant W387F/Y170W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.1 | - |
ATP | mutant W387F/Y226W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.11 | - |
ATP | mutant W387F, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.11 | - |
ATP | mutant W387F/Y371W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.11 | - |
ATP | mutant W387F/Y72W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.13 | - |
ATP | mutant W387F/Y120W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.13 | - |
L-methionine | mutant W387F/Y323W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.13 | - |
L-methionine | mutant W387F/Y72W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.18 | - |
ATP | mutant W387F/Y49W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.19 | - |
ATP | mutant W387F/Y255W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.19 | - |
ATP | wild-type, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.2 | - |
ATP | mutant W387F/Y233W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.2 | - |
ATP | mutant W387F/Y267W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.21 | - |
ATP | mutant W387F/Y85W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.22 | - |
ATP | mutant W387F/Y323W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.22 | - |
ATP | mutant W387F/Y344W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.24 | - |
ATP | mutant W387F/Y273W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.25 | - |
L-methionine | mutant W387F/Y371W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.3 | - |
L-methionine | mutant W387F/Y226W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.3 | - |
L-methionine | mutant W387F/Y273W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.31 | - |
L-methionine | mutant W387F, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.31 | - |
L-methionine | wild-type, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.33 | - |
L-methionine | mutant W387F/Y344W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.45 | - |
L-methionine | mutant W387F/Y267W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.47 | - |
L-methionine | mutant W387F/Y233W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.5 | - |
L-methionine | mutant W387F/Y255W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.54 | - |
L-methionine | mutant W387F/Y120W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.57 | - |
L-methionine | mutant W387F/Y85W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.66 | - |
L-methionine | mutant W387F/Y49W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 1 | - |
L-methionine | mutant W387F/Y170W, 55°C | Methanocaldococcus jannaschii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.6 | Methanocaldococcus jannaschii | Q58605 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.6 | ATP + L-methionine + H2O | - |
Methanocaldococcus jannaschii | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.6 | More | high stability of Methanococcus jannaschii enzyme derives largely from a tight association of its subunits in the dimer. The least stable structural elements are the C-terminal ends of beta-strands E2 and E6, and the N-terminus of E3 | Methanocaldococcus jannaschii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.6 | 1 | - |
L-methionine | mutant W387F/Y49W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 1.46 | - |
L-methionine | wild-type, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 1.54 | - |
L-methionine | mutant W387F/Y273W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 1.62 | - |
L-methionine | mutant W387F/Y323W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 1.62 | - |
L-methionine | mutant W387F/Y72W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 1.85 | - |
L-methionine | mutant W387F, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 2.31 | - |
L-methionine | mutant W387F/Y267W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 2.39 | - |
L-methionine | mutant W387F/Y170W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 2.39 | - |
L-methionine | mutant W387F/Y255W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 2.77 | - |
L-methionine | mutant W387F/Y85W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 2.85 | - |
L-methionine | mutant W387F/Y344W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 3.31 | - |
L-methionine | mutant W387F/Y120W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 4 | - |
L-methionine | mutant W387F/Y371W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 5.08 | - |
L-methionine | mutant W387F/Y226W, 55°C | Methanocaldococcus jannaschii | |
2.5.1.6 | 7.24 | - |
L-methionine | mutant W387F/Y233W, 55°C | Methanocaldococcus jannaschii |