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Literature summary extracted from

  • Wong, F.T.; Chen, A.Y.; Cane, D.E.; Khosla, C.
    Protein-protein recognition between acyltransferases and acyl carrier proteins in multimodular polyketide synthases (2010), Biochemistry, 49, 95-102.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.94 the acyltransferase domains, AT1, AT2, AT4, AT5 and AT6, from DEBS are expressed as stand-alone proteins with their ketosynthase-to-acyltransferase and post-acyltransferase linkers, ketosynthase3-acyltransferase3 and ketosynthase6-acyltransferase6 didomains and discrete acyl carrier protein domain expression vectors are constructed, the vector pET28a is used for expression in Escherichia coli BL21DE3 cells Saccharopolyspora erythraea

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.94
-
 Saccharopolyspora erythraea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.94 propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH + 6 H+ Saccharopolyspora erythraea
-
 6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.94 Saccharopolyspora erythraea Q03131
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.94
-
 Saccharopolyspora erythraea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.94 propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH + 6 H+
-
 Saccharopolyspora erythraea 6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.94 More DEBS is organized into modules comprising of multiple catalytic domains, to assemble short acyl-CoA precursors into complex polyketide products, each module contains minimally three functional domains, a beta-ketosynthase, an acyltransferase, and an acyl carrier protein Saccharopolyspora erythraea

Synonyms

EC Number Synonyms Comment Organism
2.3.1.94 6-deoxyerythronolide B synthase
-
 Saccharopolyspora erythraea
2.3.1.94 DEBS
-
 Saccharopolyspora erythraea