EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.2.43 | C148A/C149A/C173S/C216S/C318S/C383S/C400S/C411S | when assayed for growth in the presence of polymyxin, the cysteine-free construct of ArnT supports growth at a level similar to that of the native protein | Salmonella enterica subsp. enterica serovar Typhimurium |
2.4.2.43 | C148S/C149S/C173S/C216S/C318S/C383S/C400S/C411S | mutant enzyme is inactive | Salmonella enterica subsp. enterica serovar Typhimurium |
2.4.2.43 | C173S/C216S/C318S/C383S/C400S/C411S | when assayed for growth in the presence of polymyxin, the cysteine-free construct of ArnT supports growth at 70% of the native protein | Salmonella enterica subsp. enterica serovar Typhimurium |
2.4.2.43 | additional information | analysis of 31 point mutations within a putative periplasmic loop of the cysteine-free ArnT protein, carried out using an in vivo growth assay coupled with expression studies, identification of the first time specific critical residues within the bacterial transferase. These critical residues fall into two categories: those that disrupt initial protein folding or membrane localization and those that fail to confer in vivo resistance to polymyxin despite being expressed to the inner membrane | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.2.43 | inner membrane | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.43 | 4-amino-4-deoxy-alpha-L-arabinopyranosyl di-trans,poly-cis-undecaprenyl phosphate + lipid IVA | Salmonella enterica subsp. enterica serovar Typhimurium | ArnT confers resistance to the antibiotic polymyxin in Salmonella typhimurium and Escherichia coli through the modification of lipid A, a major component of the outer surface of Gram-negative bacteria. ArnT transfers a neutral aminoarabinose moiety onto the negative phosphate groups of lipid A, reducing the surface charge of the bacteria and preventing cationic peptides such as polymyxin from electrostatically recognizing and killing the bacteria. Only small amounts of ArnT are necessary to provide resistance against polymyxin to the bacterial cell | lipid IIA + di-trans,poly-cis-undecaprenyl phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.43 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.43 | 4-amino-4-deoxy-alpha-L-arabinopyranosyl di-trans,poly-cis-undecaprenyl phosphate + lipid IVA | - |
Salmonella enterica subsp. enterica serovar Typhimurium | lipid IIA + di-trans,poly-cis-undecaprenyl phosphate | - |
? | |
2.4.2.43 | 4-amino-4-deoxy-alpha-L-arabinopyranosyl di-trans,poly-cis-undecaprenyl phosphate + lipid IVA | ArnT confers resistance to the antibiotic polymyxin in Salmonella typhimurium and Escherichia coli through the modification of lipid A, a major component of the outer surface of Gram-negative bacteria. ArnT transfers a neutral aminoarabinose moiety onto the negative phosphate groups of lipid A, reducing the surface charge of the bacteria and preventing cationic peptides such as polymyxin from electrostatically recognizing and killing the bacteria. Only small amounts of ArnT are necessary to provide resistance against polymyxin to the bacterial cell | Salmonella enterica subsp. enterica serovar Typhimurium | lipid IIA + di-trans,poly-cis-undecaprenyl phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.43 | More | the secondary structure of ArnT is not dependent on disulfide bridges, Salmonella typhimurium ArnT contains no disulfide bonds | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.43 | arnT | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.2.43 | physiological function | ArnT confers resistance to the antibiotic polymyxin in Salmonella typhimurium and Escherichia coli through the modification of lipid A, a major component of the outer surface of Gram-negative bacteria. ArnT transfers a neutral aminoarabinose moiety onto the negative phosphate groups of lipid A, reducing the surface charge of the bacteria and preventing cationic peptides such as polymyxin from electrostatically recognizing and killing the bacteria. Only small amounts of ArnT are necessary to provide resistance against polymyxin to the bacterial cell | Salmonella enterica subsp. enterica serovar Typhimurium |