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Literature summary extracted from

  • Kurz, L.C.; Constantine, C.Z.; Jiang, H.; Kappock, T.J.
    The partial substrate dethiaacetyl-coenzyme A mimics all critical carbon acid reactions in the condensation half-reaction catalyzed by Thermoplasma acidophilum citrate synthase (2009), Biochemistry, 48, 7878-7891.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.16
-
 Thermoplasma acidophilum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.16 D12N reference for pI-value analysis Thermoplasma acidophilum
2.3.3.16 D317G D317 removes the acetyl-CoA methyl proton during catalysis Thermoplasma acidophilum
2.3.3.16 D317N D317 removes the acetyl-CoA methyl proton during catalysis Thermoplasma acidophilum
2.3.3.16 H187Q active site Thermoplasma acidophilum
2.3.3.16 H222Q active site Thermoplasma acidophilum
2.3.3.16 H262Q active site Thermoplasma acidophilum
2.3.3.16 W245F/W115F/W17F containing a single W (W348) Thermoplasma acidophilum
2.3.3.16 W348Y primary emitter in fluorescence analysis Thermoplasma acidophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 dethiaacetyl-CoA partial substrate Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Thermoplasma acidophilum P21553
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 oxaloacetate + acetyl-CoA + H2O
-
 Thermoplasma acidophilum citrate + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.16 citrate synthase
-
 Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.16 0.0001
-
 acetyl-CoA D317N mutant protein, value is very uncertain Thermoplasma acidophilum
2.3.3.16 0.0001
-
 oxaloacetate D317N mutant protein, value is very uncertain Thermoplasma acidophilum
2.3.3.16 0.0015
-
 acetyl-CoA D317G mutant protein Thermoplasma acidophilum
2.3.3.16 0.0015
-
 oxaloacetate D317G mutant protein Thermoplasma acidophilum
2.3.3.16 9.2
-
 acetyl-CoA wild-type protein Thermoplasma acidophilum
2.3.3.16 9.2
-
 oxaloacetate wild-type protein Thermoplasma acidophilum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.3.16 Thermoplasma acidophilum W245F/W115F/W17F mutant protein, isoelectric focusing
-
 8.6
2.3.3.16 Thermoplasma acidophilum D317G mutant protein, isoelectric focusing
-
 8.8
2.3.3.16 Thermoplasma acidophilum D317N mutant protein, isoelectric focusing
-
 8.8
2.3.3.16 Thermoplasma acidophilum wild-type protein, isoelectric focusing
-
 8.9
2.3.3.16 Thermoplasma acidophilum D12N mutant protein, isoelectric focusing
-
 9.1