EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.65 | expressed in Escherichia coli as a His-tagged fusion protein | Mycobacterium tuberculosis |
2.5.1.113 | expressed in Escherichia coli B834(DE3) and BL21(DE3) cells | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.65 | the structure of the protein complex CysM-CysO is determined at 1.53 A resolution. The protein complex in the crystal structure is asymmetric with one CysO (sulfur carrier protein) protomer binding to one end of a CysM dimer. The structures of CysM is determined individually at 2.8 A resolution. Sequence alignments with homologues and structural comparisons with CysK, a cysteine synthase that does not utilize a sulfur carrier protein, reveal high conservation of active site residues, but residues in CysM responsible for CysO binding are not conserved | Mycobacterium tuberculosis |
2.5.1.113 | native enzyme bound to CysO sulfur-carrier protein, hanging drop vapor diffusion method, using 7-10% PEG (w/v) 4000, 0.1 M sodium citrate, pH 5.8, and 0.2 M ammonium acetate at 22°C | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.65 | K204A | to improve crystallization of CysM alone, a putative surface residue in CysM (Lys204) is mutated to alanine using site-directed mutagenesis | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.113 | O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | Mycobacterium tuberculosis | - |
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate | - |
? | |
2.5.1.113 | O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | Mycobacterium tuberculosis H37Rv | - |
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.65 | Mycobacterium tuberculosis | - |
- |
- |
2.5.1.113 | Mycobacterium tuberculosis | - |
- |
- |
2.5.1.113 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.65 | using Ni-NTA chromatography | Mycobacterium tuberculosis |
2.5.1.113 | Ni-NTA column chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.113 | O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | - |
Mycobacterium tuberculosis | [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate | - |
? | |
2.5.1.113 | O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | - |
Mycobacterium tuberculosis H37Rv | [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.65 | dimer | crystal structure | Mycobacterium tuberculosis |
2.5.1.113 | homodimer | x-ray crystallography | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.65 | CysM | - |
Mycobacterium tuberculosis |
2.5.1.65 | Rv1336 | - |
Mycobacterium tuberculosis |
2.5.1.113 | CysM | - |
Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.65 | pyridoxal 5'-phosphate | - |
Mycobacterium tuberculosis | |
2.5.1.113 | pyridoxal 5'-phosphate | - |
Mycobacterium tuberculosis |