Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhang, Y.W.; Li, X.Y.; Koyama, T.
    Chain length determination of prenyltransferases: both heteromeric subunits of medium-chain (E)-prenyl diphosphate synthase are involved in the product chain length determination (2000), Biochemistry, 39, 12717-12722.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.30 A79F mutant enzyme subunit-I(wild-type)/subunit-B(A79F). Comparable kinetic constants with those of the wild-type enzyme. Significantly different pattern of product distribution from that of the wild type. The major product is hexaprenyl diphosphate Bacillus subtilis
2.5.1.30 A79L mutant enzyme subunit-I(wild-type)/subunit-B(A79L). Comparable kinetic constants with those of the wild-type enzyme. Significantly different pattern of product distribution from that of the wild type. The major product is hexaprenyl diphosphate Bacillus subtilis
2.5.1.30 D97A mutant enzyme subunit-I(D97A)/subunit-II(wild-type), major products: geranylgeranyl diphosphate, farnesylgeranyl diphosphate, hexaprenyl diphosphate, heptaprenyl diphosphate Bacillus subtilis
2.5.1.30 D97A/A79F mutant enzyme subunit-I(97A)/subunit-II(A79F) forms exclusively geranylgeranyl diphosphate Bacillus subtilis
2.5.1.30 D97A/A79L mutant enzyme subunit-I(D97A)/subunit-II(A79L), the final product is farnesylgeranyl diphosphate, increase in the production of geranylgeranyl diphosphate Bacillus subtilis
2.5.1.30 additional information several amino acid residues in the larger subunits Bacillus subtilis heptaprenyl diphosphate synthase are selected for substitutions by site-directed mutagenesis and examined by combination with the corresponding wild type or mutated smaller subunits Bacillus subtilis
2.5.1.30 V76I mutant enzyme subunit-I(wild-type)/subunit-II(A79L), octaprenyl diphosphate is the final product with farnesyl diphosphate as allylic primer Bacillus subtilis
2.5.1.30 Y103S mutant enzyme subunit-I(Y103S)/subunit-II(wild-type), final product is octaprenyl diphosphate Bacillus subtilis
2.5.1.83 A79F mutant enzyme subunit-A(wild-type)/subunit-B(A79L) shows 10fold increased Vmax-values and 11fold decreased Km-values for geranyl diphosphate, which becomes the most preferred substrate of the allylic primers. 5fold increase in KM-value for geranylgeranyl diphosphate. Mutation results in shortening the chain length of the major product. The major products are farnesylgeranyl diphosphate and geranylgeranyl diphosphate Micrococcus luteus
2.5.1.83 A79L mutant enzyme subunit-A(wild-type)/subunit-B(A79L) shows 7fold increased Vmax-values and 6fold decreased Km-values for geranyl diphosphate, which becomes the most preferred substrate of the allylic primers. 3.9fold increase in KM-value for geranylgeranyl diphosphate. Mutation results in shortening the chain length of the major product. The major product is farnesylgeranyl diphosphate Micrococcus luteus
2.5.1.83 additional information several amino acid residues in the larger subunits Bacillus subtilis heptaprenyl diphosphate synthase are selected for substitutions by site-directed mutagenesis and examined by combination with the corresponding wild type or mutated smaller subunits Micrococcus luteus
2.5.1.83 V76G mutant enzyme subunit-A(wild-type)/subunit-B(V76G) gives octaprenyl diphosphate as the final products with farnesyl diphosphate as an allylic primer Micrococcus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.30 0.0077
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C, wild-type enzyme Bacillus subtilis
2.5.1.30 0.0085
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-B(A79F) Bacillus subtilis
2.5.1.30 0.0116
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-B(I76G) Bacillus subtilis
2.5.1.30 0.0124
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79L) Bacillus subtilis
2.5.1.30 0.0143
-
 isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79F) Bacillus subtilis
2.5.1.30 0.0168
-
 geranylgeranyl diphosphate pH 8.5, 37°C, wild-type enzyme Bacillus subtilis
2.5.1.30 0.0198
-
 isopentenyl diphosphate pH 8.5, 37°C, wild-type enzyme Bacillus subtilis
2.5.1.30 0.0198
-
 geranylgeranyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(I76G) Bacillus subtilis
2.5.1.30 0.0214
-
 isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(I76G) Bacillus subtilis
2.5.1.30 0.0259
-
 isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79L) Bacillus subtilis
2.5.1.30 0.0344
-
 geranylgeranyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79L) Bacillus subtilis
2.5.1.30 0.0568
-
 geranylgeranyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79F) Bacillus subtilis
2.5.1.83 0.0067
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79F) Micrococcus luteus
2.5.1.83 0.0075
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79L) Micrococcus luteus
2.5.1.83 0.0089
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C, wild-type enzyme Micrococcus luteus
2.5.1.83 0.0105
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(V76G) Micrococcus luteus
2.5.1.83 0.0124
-
 geranylgeranyl diphosphate pH 8.5, 37°C, wild-type enzyme Micrococcus luteus
2.5.1.83 0.0137
-
 isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(V76G) Micrococcus luteus
2.5.1.83 0.0184
-
 isopentenyl diphosphate pH 8.5, 37°C, wild-type enzyme Micrococcus luteus
2.5.1.83 0.0186
-
 isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79F) Micrococcus luteus
2.5.1.83 0.0216
-
 isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79L) Micrococcus luteus
2.5.1.83 0.0216
-
 geranylgeranyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(V76G) Micrococcus luteus
2.5.1.83 0.0478
-
 geranylgeranyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79L) Micrococcus luteus
2.5.1.83 0.385
-
 geranylgeranyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79F) Micrococcus luteus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.30 Bacillus subtilis P31112 and P31114 P31112: subunit 1, P31114: subunit 2
-
2.5.1.83 Micrococcus luteus O66127 and O66129 O66127: component A of hexaprenyl diphosphate synthase, O66129: component B of hexaprenyl diphosphate synthase
-
2.5.1.83 Micrococcus luteus B-P 26 O66127 and O66129 O66127: component A of hexaprenyl diphosphate synthase, O66129: component B of hexaprenyl diphosphate synthase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.30 (2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate
-
 Bacillus subtilis 3 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.30 geranyl diphosphate + 4 isopentenyl diphosphate geranyl diphosphate is a poor substrate Bacillus subtilis 4 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.30 geranylgeranyl diphosphate + 2 isopentenyl diphosphate
-
 Bacillus subtilis 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.83 (2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate
-
 Micrococcus luteus 3 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.83 (2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate
-
 Micrococcus luteus B-P 26 3 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.83 geranyl diphosphate + 4 isopentenyl diphosphate geranyl diphosphate is a poor substrate Micrococcus luteus 4 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.83 geranyl diphosphate + 4 isopentenyl diphosphate geranyl diphosphate is a poor substrate Micrococcus luteus B-P 26 4 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.83 geranylgeranyl diphosphate + 2 isopentenyl diphosphate
-
 Micrococcus luteus 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.83 geranylgeranyl diphosphate + 2 isopentenyl diphosphate
-
 Micrococcus luteus B-P 26 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.30 HepPP synthase
-
 Bacillus subtilis
2.5.1.83 HexPP synthase
-
 Micrococcus luteus