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Literature summary extracted from

  • Eprintsev, A.T.; Maslova, E.V.; Fedorin, D.N.; Popov, V.N.
    Physicochemical and kinetic characteristics of isoforms of isocitrate lyase from corn (2009), Biochemistry (Moscow), 74, 528-532.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.056
-
isocitrate ICL1 Zea mays
4.1.3.1 0.083
-
isocitrate ICL2 Zea mays
4.1.3.1 0.1
-
isocitrate ICL Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Mg2+ ICL1, dependent on Zea mays
4.1.3.1 Mn2+ ICL2 and ICL, dependent on Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 164000
-
gel filtration, ICL1 Zea mays
4.1.3.1 207000
-
gel filtration, ICL2 Zea mays
4.1.3.1 208000
-
gel filtration, ICL Zea mays

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1 to homogeneity, purification of ICL1 is 123fold with a yield of 5.7%, purification degree and yield of ICL2 are 110 and 5.7%, respectively Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.1 leaf ICL Zea mays
-
4.1.3.1 scutellum ICL1 and and ICL2 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 0.04
-
homogenate Zea mays
4.1.3.1 4.4
-
ICL2, 1003fold purified Zea mays
4.1.3.1 4.9
-
ICL1, 123fold purified Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate ICL1, ICL2 and ICL Zea mays succinate + glyoxylate
-
?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 tetramer gel filtration, ICL1, ICL2 and ICL Zea mays

Synonyms

EC Number Synonyms Comment Organism
4.1.3.1 ICL
-
Zea mays
4.1.3.1 isocitrate lyase
-
Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.1 6
-
ICL2 Zea mays
4.1.3.1 7.5
-
ICL1 Zea mays

Expression

EC Number Organism Comment Expression
4.1.3.1 Zea mays Ca2+, K+, and Na+ have little effect on the regulation of activity of ICL isoforms additional information

General Information

EC Number General Information Comment Organism
4.1.3.1 metabolism participation of isoforms in metabolic regulation of the glyoxylate cycle, organic acid metabolism during photorespiration in leaves and acidosis in corn seeds Zea mays