EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.1 | 0.056 | - |
isocitrate | ICL1 | Zea mays | |
4.1.3.1 | 0.083 | - |
isocitrate | ICL2 | Zea mays | |
4.1.3.1 | 0.1 | - |
isocitrate | ICL | Zea mays |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.3.1 | Mg2+ | ICL1, dependent on | Zea mays | |
4.1.3.1 | Mn2+ | ICL2 and ICL, dependent on | Zea mays |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.3.1 | 164000 | - |
gel filtration, ICL1 | Zea mays |
4.1.3.1 | 207000 | - |
gel filtration, ICL2 | Zea mays |
4.1.3.1 | 208000 | - |
gel filtration, ICL | Zea mays |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.3.1 | Zea mays | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.3.1 | to homogeneity, purification of ICL1 is 123fold with a yield of 5.7%, purification degree and yield of ICL2 are 110 and 5.7%, respectively | Zea mays |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.1.3.1 | leaf | ICL | Zea mays | - |
4.1.3.1 | scutellum | ICL1 and and ICL2 | Zea mays | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.3.1 | 0.04 | - |
homogenate | Zea mays |
4.1.3.1 | 4.4 | - |
ICL2, 1003fold purified | Zea mays |
4.1.3.1 | 4.9 | - |
ICL1, 123fold purified | Zea mays |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.1 | isocitrate | ICL1, ICL2 and ICL | Zea mays | succinate + glyoxylate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.3.1 | tetramer | gel filtration, ICL1, ICL2 and ICL | Zea mays |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.3.1 | ICL | - |
Zea mays |
4.1.3.1 | isocitrate lyase | - |
Zea mays |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.3.1 | 6 | - |
ICL2 | Zea mays |
4.1.3.1 | 7.5 | - |
ICL1 | Zea mays |
EC Number | Organism | Comment | Expression |
---|---|---|---|
4.1.3.1 | Zea mays | Ca2+, K+, and Na+ have little effect on the regulation of activity of ICL isoforms | additional information |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.3.1 | metabolism | participation of isoforms in metabolic regulation of the glyoxylate cycle, organic acid metabolism during photorespiration in leaves and acidosis in corn seeds | Zea mays |