EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.18 | ascorbate | addition of a second electron donor like ascorbate leads to 10times faster heme conversion | Arabidopsis thaliana | |
1.14.14.18 | additional information | heme turnover is promoted by light when spinach thylakoids are present | Arabidopsis thaliana | |
1.14.15.20 | L-ascorbate | adding of a second electron donor, such as ascorbate, led to a 10fold increase in the heme conversion rate | Arabidopsis thaliana |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.18 | HY1, HO3, and HO4 expressed without their predicted chloroplast transit peptides either using a tac promoter-driven N-terminal GST fusion expression system (HY1) or the pET vector system (HO3, HO4) using a T7-promoter and a His-tag fusion. Plasmids pGEX-mHY1, pET24a-mHO3 and pET24a-mHO4 expressed in Escherichia coli BL21 (DE3). Fluorescent protein expression in Nicotiana benthamiana by using Agrobacterium tumefaciens C58C1 pGV2260 | Arabidopsis thaliana |
1.14.15.20 | expressed in Nicotiana benthamiana and Escherichia coli BL21 (DE3) cells | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.18 | 0.0027 | - |
heme | HO3, without ascorbate | Arabidopsis thaliana | |
1.14.14.18 | 0.0057 | - |
heme | HO4, without ascorbate | Arabidopsis thaliana | |
1.14.15.20 | 0.0013 | - |
protoheme | isoform HY1, at pH 7.2 and 25°C | Arabidopsis thaliana | |
1.14.15.20 | 0.0027 | - |
protoheme | isoform HO3, at pH 7.2 and 25°C | Arabidopsis thaliana | |
1.14.15.20 | 0.0057 | - |
protoheme | isoform HO4, at pH 7.2 and 25°C | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.18 | chloroplast | HY1, HO3, and HO4 are present as the processed mature protein in the plastid compartment | Arabidopsis thaliana | 9507 | - |
1.14.14.18 | additional information | no mitochondrial localization of HY1, HO3, and HO4 | Arabidopsis thaliana | - |
- |
1.14.15.20 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
1.14.15.20 | nucleus | - |
Arabidopsis thaliana | 5634 | - |
1.14.15.20 | plastid | isoforms HY1, HO3 and HO4 | Arabidopsis thaliana | 9536 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.18 | 27000 | - |
1 * 27000, HY1, SDS-PAGE. HY1, HO3 and HO4, gel filtration | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.20 | protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Arabidopsis thaliana | - |
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.18 | Arabidopsis thaliana | - |
- |
- |
1.14.15.20 | Arabidopsis thaliana | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.18 | HY1, HO3, and HO4. HO3 and HO4 purified by cobalt affinity chromatography, to over 90% purity | Arabidopsis thaliana |
1.14.15.20 | Talon-cobalt-affinity column chromatography | Arabidopsis thaliana |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.14.18 | 0°C, 100 mM potassium phosphate buffer, pH 7.2, 4 days | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.18 | heme + ferredoxin + O2 | HY1, HO3, and HO4 | Arabidopsis thaliana | biliverdin IXalpha + Fe2+ + CO + A + H2O | - |
? | |
1.14.15.20 | protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Arabidopsis thaliana | biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.18 | monomer | 1 * 27000, HY1, SDS-PAGE. HY1, HO3 and HO4, gel filtration | Arabidopsis thaliana |
1.14.15.20 | monomer | - |
Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.18 | Ho3 | - |
Arabidopsis thaliana |
1.14.14.18 | HO4 | - |
Arabidopsis thaliana |
1.14.14.18 | HY1 | HO1 | Arabidopsis thaliana |
1.14.15.20 | haem oxygenase | - |
Arabidopsis thaliana |
1.14.15.20 | Ho1 | - |
Arabidopsis thaliana |
1.14.15.20 | Ho3 | - |
Arabidopsis thaliana |
1.14.15.20 | HO4 | - |
Arabidopsis thaliana |
1.14.15.20 | HY1 | - |
Arabidopsis thaliana |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.18 | 10 | 45 | - |
Arabidopsis thaliana |
1.14.15.20 | 10 | 45 | the relative enzyme activity increases between 10°C and 45°C | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.18 | 7 | - |
HO3 | Arabidopsis thaliana |
1.14.14.18 | 7.25 | - |
HY1 and HO4 | Arabidopsis thaliana |
1.14.15.20 | 7 | - |
isoform HO3 | Arabidopsis thaliana |
1.14.15.20 | 7.3 | - |
isoforms HO4 and HY1 | Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.18 | heme | - |
Arabidopsis thaliana | |
1.14.14.18 | NADPH | - |
Arabidopsis thaliana |