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Literature summary extracted from

  • Roch, C.; Kuhn, J.; Kleesiek, K.; Goetting, C.
    Differences in gene expression of human xylosyltransferases and determination of acceptor specificities for various proteoglycans (2010), Biochem. Biophys. Res. Commun., 391, 685-691.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.26 expressed as glutathione-S-transferase fusion proteins containing putative or known GAG attachment sites of in vivo proteoglycans. Cloned into the XhoI and EcoRI digested pGEX-6P-1 vector and ligated by T4 DNA ligase. Expressed in Escherichia coli BL21 DE3 cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.26 0.0023
-
 serglycin isoenzyme XT-II Homo sapiens
2.4.2.26 0.0029
-
 Thrombomodulin isoenzyme XT-II Homo sapiens
2.4.2.26 0.0032
-
 neuroglycan C isoenzyme XT-II Homo sapiens
2.4.2.26 0.0034
-
 collagen alpha2(IX) isoenzyme XT-I Homo sapiens
2.4.2.26 0.0035
-
 syndecan-4 isoenzyme XT-I Homo sapiens
2.4.2.26 0.0064
-
 bikunin isoenzyme XT-II with mutant bikunin (sequence QEE-GSGRGQLV) Homo sapiens
2.4.2.26 0.0074
-
 syndecan-1 isoenzyme XT-II Homo sapiens
2.4.2.26 0.0077
-
 bikunin isoenzyme XT-II with mutant bikunin (sequence QEEEGSGHNMLV) Homo sapiens
2.4.2.26 0.0082
-
 testican-2 isoenzyme XT-II Homo sapiens
2.4.2.26 0.0085
-
 bikunin isoenzyme XT-II with mutant bikunin (sequence QEEESSGGGQLV) Homo sapiens
2.4.2.26 0.0093
-
 bFGF-peptide isoenzyme XT-II Homo sapiens
2.4.2.26 0.0093
-
 bikunin isoenzyme XT-II with mutant bikunin (sequence QEEEGSGSLALV) Homo sapiens
2.4.2.26 0.0094
-
 perlecan-3 isoenzyme XT-II Homo sapiens
2.4.2.26 0.0097
-
 biglycan isoenzyme XT-I Homo sapiens
2.4.2.26 0.0099
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEEERSGGGQLV) Homo sapiens
2.4.2.26 0.0102
-
 perlecan-2 isoenzyme XT-II Homo sapiens
2.4.2.26 0.0103
-
 testican-2 isoenzyme XT-I Homo sapiens
2.4.2.26 0.0103
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEEESSGGGQLV) Homo sapiens
2.4.2.26 0.0104
-
 L-APLP2 isoenzyme XT-II Homo sapiens
2.4.2.26 0.0109
-
 glypican-1 isoenzyme XT-II Homo sapiens
2.4.2.26 0.0111
-
 serglycin isoenzyme XT-I Homo sapiens
2.4.2.26 0.0122
-
 syndecan isoenzyme XT-II Homo sapiens
2.4.2.26 0.0123
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEE-GSGGGQLV) Homo sapiens
2.4.2.26 0.0125
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEEEGSGHNMLV) Homo sapiens
2.4.2.26 0.0127
-
 versican-beta isoenzyme XT-II Homo sapiens
2.4.2.26 0.0131
-
 L-APP XT-I Homo sapiens
2.4.2.26 0.0133
-
 perlecan-1 isoenzyme XT-I Homo sapiens
2.4.2.26 0.0133
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEEEGSSCGQLV) Homo sapiens
2.4.2.26 0.0134
-
 L-APLP2 isoenzyme XT-I Homo sapiens
2.4.2.26 0.0137
-
 bFGF-peptide isoenzyme XT-I Homo sapiens
2.4.2.26 0.0137
-
 bikunin isoenzyme XT-II with mutant bikunin (sequence QEEEGSGRRALV) Homo sapiens
2.4.2.26 0.0141
-
 syndecan-4 isoenzyme XT-II Homo sapiens
2.4.2.26 0.0145
-
 perlecan-2 isoenzyme XT-I Homo sapiens
2.4.2.26 0.0146
-
 CD44 isoenzyme XT-I Homo sapiens
2.4.2.26 0.0146
-
 versican-beta isoenzyme XT-I Homo sapiens
2.4.2.26 0.015
-
 bikunin isoenzyme XT-II with mutant bikunin (sequence QEEEGSGGRSVLV) Homo sapiens
2.4.2.26 0.0151
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEEEGSGCGQLV) Homo sapiens
2.4.2.26 0.0153
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEEEGSSGGQLV) Homo sapiens
2.4.2.26 0.0166
-
 phosphacan isoenzyme XT-I Homo sapiens
2.4.2.26 0.0171
-
 glypican-1 isoenzyme XT-I Homo sapiens
2.4.2.26 0.0178
-
 syndecan isoenzyme XT-I Homo sapiens
2.4.2.26 0.0179
-
 bamcan isoenzyme XT-II Homo sapiens
2.4.2.26 0.0182
-
 Thrombomodulin isoenzyme XT-I Homo sapiens
2.4.2.26 0.0187
-
 perlecan-3 isoenzyme XT-I Homo sapiens
2.4.2.26 0.0188
-
 Aggrecan isoenzyme XT-I Homo sapiens
2.4.2.26 0.0198
-
 syndecan-1 isoenzyme XT-I Homo sapiens
2.4.2.26 0.0199
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEEEGSGSLALV) Homo sapiens
2.4.2.26 0.02
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QKEEGSGGGQLV) Homo sapiens
2.4.2.26 0.0205
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEE-GSGRGQLV) Homo sapiens
2.4.2.26 0.0208
-
 betaglycan-1 isoenzyme XT-I Homo sapiens
2.4.2.26 0.0215
-
 bikunin isoenzyme XT-II with mutant bikunin (sequence QEEEGSGCYKLV) Homo sapiens
2.4.2.26 0.0222
-
 bamcan isoenzyme XT-I Homo sapiens
2.4.2.26 0.0227
-
 bikunin isoenzyme XT-II with mutant bikunin (sequence QEEEGSGCGQLV) Homo sapiens
2.4.2.26 0.0236
-
 neuroglycan C isoenzyme XT-I Homo sapiens
2.4.2.26 0.0242
-
 bikunin isoenzyme XT-II with mutant bikunin (sequence QEEEGSGRHLLV) Homo sapiens
2.4.2.26 0.0243
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEEESSGRGQLV) Homo sapiens
2.4.2.26 0.0251
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEEEGSGGRSVLV) Homo sapiens
2.4.2.26 0.0274
-
 bikunin isoenzyme XT-I with wild-type bikunin (sequence QEEEGSGGGQLV) Homo sapiens
2.4.2.26 0.0288
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEEEGSTSLV) Homo sapiens
2.4.2.26 0.0301
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEEEGSGCYKLV) Homo sapiens
2.4.2.26 0.0318
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QLTTGSGGGQLV) Homo sapiens
2.4.2.26 0.0356
-
 bikunin isoenzyme XT-II with mutant bikunin (sequence QKEEGSGGGQLV) Homo sapiens
2.4.2.26 0.0462
-
 bikunin isoenzyme XT-II with wild-type bikunin (sequence QEEEGSGGGQLV) Homo sapiens
2.4.2.26 0.0463
-
 bikunin isoenzyme XT-II Homo sapiens
2.4.2.26 0.0508
-
 bikunin isoenzyme XT-II with mutant bikunin (sequence QEEEGSGCRVLV) Homo sapiens
2.4.2.26 0.0543
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEEEGSGCRVLV) Homo sapiens
2.4.2.26 0.0572
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEEEGSGRHLLV) Homo sapiens
2.4.2.26 0.0586
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QEEEGSGRRALV) Homo sapiens
2.4.2.26 0.0656
-
 bikunin isoenzyme XT-I with mutant bikunin (sequence QPQIGSGGGQLV) Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.26 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.26 by affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.26 A-431 cell XT-I mRNA is not expressed but XT-II mRNA Homo sapiens
-
2.4.2.26 A-549 cell XT-II mRNA expression is higher than for XT-I. Very low XT-I mRNA expression Homo sapiens
-
2.4.2.26 BM-1604 cell XT-I mRNA is not expressed but XT-II mRNA Homo sapiens
-
2.4.2.26 BT-474 cell highest XT-II expression levels, low XT-I mRNA expression Homo sapiens
-
2.4.2.26 CAPAN-1 cell XT-II mRNA expression is higher than for XT-I Homo sapiens
-
2.4.2.26 CAPAN-2 cell XT-I mRNA is not expressed but XT-II mRNA Homo sapiens
-
2.4.2.26 Chang cell XT-II mRNA expression is higher than for XT-I. Very low XT-I mRNA expression Homo sapiens
-
2.4.2.26 EAhy 926 cell XT-II mRNA expression is higher than for XT-I. Very low XT-I mRNA expression Homo sapiens
-
2.4.2.26 ECV-304 cell XT-II mRNA expression is higher than for XT-I. Very low XT-I mRNA expression Homo sapiens
-
2.4.2.26 EFO-27 cell XT-II mRNA expression is higher than for XT-I. Low XT-I mRNA expression Homo sapiens
-
2.4.2.26 HEK-293 cell XT-II mRNA expression is higher than for XT-I. Very low XT-I mRNA expression Homo sapiens
-
2.4.2.26 HeLa cell XT-I mRNA is not expressed but XT-II mRNA Homo sapiens
-
2.4.2.26 Hep-G2 cell XT-II mRNA expression is higher than for XT-I. Very low XT-I mRNA expression Homo sapiens
-
2.4.2.26 HT-1080 cell XT-I mRNA is not expressed but XT-II mRNA Homo sapiens
-
2.4.2.26 JAR cell XT-I mRNA expression is higher than for XT-II Homo sapiens
-
2.4.2.26 K-562 cell XT-I mRNA is not expressed but XT-II mRNA Homo sapiens
-
2.4.2.26 LNCaP cell XT-I mRNA is not expressed but XT-II mRNA Homo sapiens
-
2.4.2.26 MCF-7 cell XT-II mRNA expression is higher than for XT-I Homo sapiens
-
2.4.2.26 additional information equal expression levels of XT-I and XT-II in 1301 cells, WERI-RB-1 cells and HS-27 cells. In 3051/80 cells, SW-982 cells, MHH-ES-1 cells, hTERT-BJ1 cells and EFO-21 cells, XT-II mRNA expression is higher than for XT-I. In 23132/87 cells, XT-I mRNA expression is higher than for XT-II. In NCI-H510A cells, highest XT-I expression levels, XT-I mRNA expression is higher than for XT-II Homo sapiens
-
2.4.2.26 RPMI-2650 cell XT-II mRNA expression is higher than for XT-I. Very low XT-I mRNA expression Homo sapiens
-
2.4.2.26 SAOS-2 cell equal expression levels of XT-I and XT-II Homo sapiens
-
2.4.2.26 SW-1353 cell equal expression levels of XT-I and XT-II Homo sapiens
-
2.4.2.26 THP-1 cell XT-I mRNA expression is higher than for XT-II Homo sapiens
-
2.4.2.26 Y-79 cell equal expression levels of XT-I and XT-II Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.26 UDP-D-xylose + aggrecan
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + bamcan
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + betaglycan-1
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + bFGF-peptide
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + biglycan
-
 Homo sapiens UDP + D-xylosyl-biglycan
-
 ?
2.4.2.26 UDP-D-xylose + bikunin
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + CD44
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + collagen alpha2(IX)
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + glypican-1
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + L-APLP2
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + L-APP
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + neuroglycan C
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + perlecan-1
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + perlecan-2
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + perlecan-3
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + phosphacan
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + serglycin
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + syndecan
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + syndecan-1
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + syndecan-4
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + testican-2
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + thrombomodulin
-
 Homo sapiens UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + versican-beta
-
 Homo sapiens UDP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.26 XT-I
-
 Homo sapiens
2.4.2.26 XT-II
-
 Homo sapiens
2.4.2.26 xylosyltransferase I
-
 Homo sapiens
2.4.2.26 xylosyltransferase II
-
 Homo sapiens