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Literature summary extracted from

  • Knecht, W.; Mikkelsen, N.E.; Clausen, A.R.; Willer, M.; Eklund, H.; Gojkovic, Z.; Piskur, J.
    Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine (2009), Biochem. Biophys. Res. Commun., 382, 430-433.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.145 truncated dNK lacking the last 20 amino acid residues in complex with gemcitabine, hanging drop vapor diffusion method, using 0.1 M MES pH 6.5, 0.2 M lithium sulfate and 26% (w/v) mPEG2000 Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.145 0.0207
-
 2'-deoxy-2',2'-difluorocytidine
-
 Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.145 Drosophila melanogaster Q9XZT6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.145 ATP + 2'-deoxy-2',2'-difluorocytidine 2'-deoxy-2',2'-difluorocytidine is gemcitabine, dNK is an activator of gemcitabine Drosophila melanogaster ADP + difluorodeoxycytidine 5'-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.145 dNK
-
 Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.145 6.5
-
 2'-deoxy-2',2'-difluorocytidine
-
 Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.145 ATP
-
 Drosophila melanogaster

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.145 314
-
 2'-deoxy-2',2'-difluorocytidine
-
 Drosophila melanogaster