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Literature summary extracted from

  • Mizanur, R.M.; Griffin, A.K.; Pohl, N.L.
    Recombinant production and biochemical characterization of a hyperthermostable alpha-glucan/maltodextrin phosphorylase from Pyrococcus furiosus (2008), Archaea, 2, 169-176.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.1 expression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 31
-
phosphate 80°C, pH 6.5 Pyrococcus furiosus
2.4.1.1 31
-
phosphate pH 6.8, 80°C Pyrococcus furiosus
2.4.1.1 70
-
maltotriose 80°C, pH 6.5, direction of glucose 1-phosphate synthesis Pyrococcus furiosus
2.4.1.1 70
-
maltotriose pH 6.8, 80°C Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 98000
-
mass spectrometry Pyrococcus furiosus
2.4.1.1 98000
-
x * 98000, SDS-PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Pyrococcus furiosus
-
-
-
2.4.1.1 Pyrococcus furiosus Q8U0Q0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1
-
Pyrococcus furiosus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate sequential bi-bi mechanism Pyrococcus furiosus
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate sequential bi-bi catalytic mechanism Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 31
-
80°C, pH 6.5, substrate glycogen Pyrococcus furiosus
2.4.1.1 52
-
80°C, pH 6.5, substrate maltoheptaose Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 glycogen + alpha-D-glucose 1-phosphate 75% of the activity with maltose, 64% of the activity with maltoheptaose Pyrococcus furiosus glycogen + phosphate
-
r
2.4.1.1 glycogen + phosphate
-
Pyrococcus furiosus ? + alpha-D-glucose 1-phosphate
-
r
2.4.1.1 maltoheptaose + alpha-D-glucose 1-phosphate 80% of the activity with maltose Pyrococcus furiosus maltooctaose + phosphate
-
r
2.4.1.1 maltoheptaose + phosphate
-
Pyrococcus furiosus maltohexaose + alpha-D-glucose 1-phosphate
-
r
2.4.1.1 maltoheptaose + phosphate 35% of the activity with maltoheptaose Pyrococcus furiosus maltohexaose + alpha-D-glucose 1-phosphate
-
r
2.4.1.1 maltohexaose + alpha-D-glucose 1-phosphate 82% of the activity with maltose Pyrococcus furiosus maltoheptaose + phosphate
-
r
2.4.1.1 maltohexaose + phosphate
-
Pyrococcus furiosus maltopentaose + alpha-D-glucose 1-phosphate
-
r
2.4.1.1 maltohexaose + phosphate 85% of the activity with maltoheptaose Pyrococcus furiosus maltopentaose + alpha-D-glucose 1-phosphate
-
r
2.4.1.1 maltopentaose + alpha-D-glucose 1-phosphate 86% of the activity with maltose Pyrococcus furiosus maltohexaose + phosphate
-
r
2.4.1.1 maltopentaose + phosphate
-
Pyrococcus furiosus maltotetraose + alpha-D-glucose 1-phosphate
-
r
2.4.1.1 maltopentaose + phosphate 78% of the activity with maltoheptaose Pyrococcus furiosus maltotetraose + alpha-D-glucose 1-phosphate
-
r
2.4.1.1 maltose + alpha-D-glucose 1-phosphate
-
Pyrococcus furiosus maltotriose + phosphate
-
r
2.4.1.1 maltotetraose + alpha-D-glucose 1-phosphate 90% of the activity with maltose Pyrococcus furiosus maltopentaose + phosphate
-
r
2.4.1.1 maltotetraose + phosphate
-
Pyrococcus furiosus maltotriose + alpha-D-glucose 1-phosphate
-
r
2.4.1.1 maltotetraose + phosphate 61% of the activity with maltoheptaose Pyrococcus furiosus maltotriose + alpha-D-glucose 1-phosphate
-
r
2.4.1.1 maltotriose + alpha-D-glucose 1-phosphate 95% of the activity with maltose Pyrococcus furiosus maltotetraose + phosphate
-
r
2.4.1.1 maltotriose + phosphate
-
Pyrococcus furiosus maltose + alpha-D-glucose 1-phosphate
-
r
2.4.1.1 maltotriose + phosphate 35% of the activity with maltoheptaose Pyrococcus furiosus maltose + alpha-D-glucose 1-phosphate
-
r
2.4.1.1 starch + phosphate
-
Pyrococcus furiosus ? + alpha-D-glucose 1-phosphate
-
r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate broad substrate specificity in both the synthesis and degradation of maltooligosaccharides In the synthetic direction of the enzymatic reaction, the lowest oligosaccharide required for the chain elongation is maltose. In the degradative direction the archaeal enzyme can produce D-glucose-1-phosphate from maltotriose or longer maltooligosaccharides including both glycogen and starch Pyrococcus furiosus [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.1 ? x * 98000, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.1 alpha-glucan/maltodextrin phosphorylase
-
Pyrococcus furiosus
2.4.1.1 PF1535 locus name Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 80
-
-
Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1 80
-
Tris-HCl buffer, 24 h, no loss of activity Pyrococcus furiosus
2.4.1.1 80
-
retains its complete activity after 24 h at 80°C in Tris-HCl buffer Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 6.5 7
-
Pyrococcus furiosus