EC Number | Cloned (Comment) | Organism |
---|---|---|
1.10.3.1 | AoCO4 is expressed in Trichoderma reesei under the strong cbh1 promoter, resulting in high yields of extracellular enzyme, corresponding to 1.5 g/l production of the enzyme | Aspergillus oryzae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 39350 | - |
MALDI-TOF | Aspergillus oryzae |
1.10.3.1 | 40480 | - |
MALDI-TOF | Aspergillus oryzae |
1.10.3.1 | 53000 | - |
SDS-PAGE | Aspergillus oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.3.1 | Aspergillus oryzae | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.10.3.1 | proteolytic modification | the N-terminal analysis of the protein reveals N-terminal processing taking place in the Kex2/furin-type protease cleavage site and removing the first 51 amino acids from the putative N-terminus | Aspergillus oryzae |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.10.3.1 | AoCO4 is purified with a two-step purification procedure, consisting of cation and anion exchange chromatography | Aspergillus oryzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.1 | 2 catechol + O2 | - |
Aspergillus oryzae | 2 1,2-benzoquinone + 2 H2O | - |
? | |
1.10.3.1 | 4-tert-butylcatechol + O2 | - |
Aspergillus oryzae | ? | - |
? | |
1.10.3.1 | aminophenol + O2 | - |
Aspergillus oryzae | ? | - |
? | |
1.10.3.1 | aniline + O2 | enzyme shows also activity after 24 h incubation | Aspergillus oryzae | ? | - |
? | |
1.10.3.1 | caffeic acid + O2 | - |
Aspergillus oryzae | ? | - |
? | |
1.10.3.1 | guaiacol + O2 | - |
Aspergillus oryzae | ? | - |
? | |
1.10.3.1 | p-cresol + O2 | enzyme shows also activity after 24 h incubation | Aspergillus oryzae | ? | - |
? | |
1.10.3.1 | phenol + O2 | enzyme shows also activity after 24 h incubation | Aspergillus oryzae | ? | - |
? | |
1.10.3.1 | tyrosol + O2 | enzyme shows also activity after 24 h incubation | Aspergillus oryzae | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.10.3.1 | AoCO4 | - |
Aspergillus oryzae |
1.10.3.1 | catechol oxidase | - |
Aspergillus oryzae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 25 | - |
assay at | Aspergillus oryzae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 50 | - |
half-live 20 h at 50°C | Aspergillus oryzae |
1.10.3.1 | 60 | - |
enzyme shows thermostability up to 60°C, half-live 2 h at 60°C | Aspergillus oryzae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 5.6 | - |
- |
Aspergillus oryzae |
1.10.3.1 | 7 | - |
assay at | Aspergillus oryzae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 5 | 7 | - |
Aspergillus oryzae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 5 | - |
cosiderable activity loss below pH 5.0 | Aspergillus oryzae |