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Literature summary extracted from

  • Gasparetti, C.; Faccio, G.; Arvas, M.; Buchert, J.; Saloheimo, M.; Kruus, K.
    Discovery of a new tyrosinase-like enzyme family lacking a C-terminally processed domain: production and characterization of an Aspergillus oryzae catechol oxidase (2009), Appl. Microbiol. Biotechnol., 86, 213-226.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.1 AoCO4 is expressed in Trichoderma reesei under the strong cbh1 promoter, resulting in high yields of extracellular enzyme, corresponding to 1.5 g/l production of the enzyme Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 39350
-
MALDI-TOF Aspergillus oryzae
1.10.3.1 40480
-
MALDI-TOF Aspergillus oryzae
1.10.3.1 53000
-
SDS-PAGE Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Aspergillus oryzae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.1 proteolytic modification the N-terminal analysis of the protein reveals N-terminal processing taking place in the Kex2/furin-type protease cleavage site and removing the first 51 amino acids from the putative N-terminus Aspergillus oryzae

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 AoCO4 is purified with a two-step purification procedure, consisting of cation and anion exchange chromatography Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 2 catechol + O2
-
Aspergillus oryzae 2 1,2-benzoquinone + 2 H2O
-
?
1.10.3.1 4-tert-butylcatechol + O2
-
Aspergillus oryzae ?
-
?
1.10.3.1 aminophenol + O2
-
Aspergillus oryzae ?
-
?
1.10.3.1 aniline + O2 enzyme shows also activity after 24 h incubation Aspergillus oryzae ?
-
?
1.10.3.1 caffeic acid + O2
-
Aspergillus oryzae ?
-
?
1.10.3.1 guaiacol + O2
-
Aspergillus oryzae ?
-
?
1.10.3.1 p-cresol + O2 enzyme shows also activity after 24 h incubation Aspergillus oryzae ?
-
?
1.10.3.1 phenol + O2 enzyme shows also activity after 24 h incubation Aspergillus oryzae ?
-
?
1.10.3.1 tyrosol + O2 enzyme shows also activity after 24 h incubation Aspergillus oryzae ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 AoCO4
-
Aspergillus oryzae
1.10.3.1 catechol oxidase
-
Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 25
-
assay at Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.1 50
-
half-live 20 h at 50°C Aspergillus oryzae
1.10.3.1 60
-
enzyme shows thermostability up to 60°C, half-live 2 h at 60°C Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 5.6
-
-
Aspergillus oryzae
1.10.3.1 7
-
assay at Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.1 5 7
-
Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.1 5
-
cosiderable activity loss below pH 5.0 Aspergillus oryzae