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Literature summary extracted from

  • Bell, S.G.; Dale, A.; Rees, N.H.; Wong, L.L.
    A cytochrome P450 class I electron transfer system from Novosphingobium aromaticivorans (2010), Appl. Microbiol. Biotechnol., 86, 163-175.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.84 CYP111A2 cloning, expression in Escherichia coli strain DH5alpha Novosphingobium aromaticivorans
1.14.15.1 ArR/Arx electron-transport chain co-expressed with the CYP enzymes in Escherichia coli. Amplified genes incorporated into the pRSFDuet-Arx vector using the Nde I and Kpn I/EcoR V restriction sites, plasmids pETDuet-Arx-ArR and one of the P450 expressing plasmids pRSFDuet-Arx-CYP transformed into Escherichia coli competent BL21(DE3) cells Novosphingobium aromaticivorans

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.84 additional information interaction and coupling of CYP enzyme reactions from Novosphingobium aromaticivorans with ferredoxin reductases ArR from Novosphingobium aromaticivorans and PdR from from Rhodopseudomonas palustris CGA009, activity reconstitution with the electron transfer system of ArR and Arx, overview Novosphingobium aromaticivorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.84 Fe2+ in the heme cofactor Novosphingobium aromaticivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Novosphingobium aromaticivorans
-
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
?
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Novosphingobium aromaticivorans ATCC 700278D-5
-
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.84 Novosphingobium aromaticivorans
-
-
-
1.14.14.84 Novosphingobium aromaticivorans ATCC 700278D-5
-
-
-
1.14.15.1 Novosphingobium aromaticivorans
-
strain ATCC 700278D-5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.84 recombinant enzyme from Escherichia coli by ultrafiltration and anion exchange chromatography Novosphingobium aromaticivorans

Storage Stability

EC Number Storage Stability Organism
1.14.15.1 -20°C, 50 mM Tris buffer, pH 7.4, 50% v/v glycerol Novosphingobium aromaticivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
Novosphingobium aromaticivorans (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
?
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
Novosphingobium aromaticivorans (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O NMR spectroscopy product analysis ?
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
Novosphingobium aromaticivorans ATCC 700278D-5 (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
?
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin CYP101D1, CYP101D2 Novosphingobium aromaticivorans (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
?
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin CYP101D1, CYP101D2 Novosphingobium aromaticivorans ATCC 700278D-5 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
?
1.14.15.1 2-adamantanone + O2 + reduced putidaredoxin CYP101D1 and CYP101D2 Novosphingobium aromaticivorans 5-hydroxy-2-adamantanone + oxidized putidaredoxin + H2O
-
?
1.14.15.1 2-adamantanone + O2 + reduced putidaredoxin CYP101D1 and CYP101D2 Novosphingobium aromaticivorans ATCC 700278D-5 5-hydroxy-2-adamantanone + oxidized putidaredoxin + H2O
-
?
1.14.15.1 beta-ionone + O2 + reduced putidaredoxin CYP101C1 and CYP101B1. CYP101C1 oxidizes beta-ionone to 4-hydroxy-beta-ionone (75%) with one other, unidentified product. This latter compound is the major product of beta-ionone oxidation by CYP101B1 (90%) where 4-hydroxy-beta-ionone is the minor product (10%) Novosphingobium aromaticivorans 4-hydroxy-beta-ionone + oxidized putidaredoxin + H2O
-
?
1.14.15.1 beta-ionone + O2 + reduced putidaredoxin CYP101C1 and CYP101B1. CYP101C1 oxidizes beta-ionone to 4-hydroxy-beta-ionone (75%) with one other, unidentified product. This latter compound is the major product of beta-ionone oxidation by CYP101B1 (90%) where 4-hydroxy-beta-ionone is the minor product (10%) Novosphingobium aromaticivorans ATCC 700278D-5 4-hydroxy-beta-ionone + oxidized putidaredoxin + H2O
-
?
1.14.15.1 linalool + O2 + reduced putidaredoxin CYP111A2 and CYP111A1 Novosphingobium aromaticivorans 8-hydroxy-linalool + oxidized putidaredoxin + H2O
-
?
1.14.15.1 linalool + O2 + reduced putidaredoxin CYP111A2 and CYP111A1 Novosphingobium aromaticivorans ATCC 700278D-5 8-hydroxy-linalool + oxidized putidaredoxin + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.84 CYP111A2
-
Novosphingobium aromaticivorans
1.14.14.84 linalool hydroxylase
-
Novosphingobium aromaticivorans
1.14.15.1 CYP101B1
-
Novosphingobium aromaticivorans
1.14.15.1 CYP101C1
-
Novosphingobium aromaticivorans
1.14.15.1 CYP101D1
-
Novosphingobium aromaticivorans
1.14.15.1 CYP101D2
-
Novosphingobium aromaticivorans
1.14.15.1 CYP111A2
-
Novosphingobium aromaticivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.84 30
-
assay at Novosphingobium aromaticivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.84 7.4
-
assay at Novosphingobium aromaticivorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.84 cytochrome P450
-
Novosphingobium aromaticivorans
1.14.14.84 heme dependent on Novosphingobium aromaticivorans
1.14.15.1 putidaredoxin
-
Novosphingobium aromaticivorans

General Information

EC Number General Information Comment Organism
1.14.14.84 evolution the enzyme belongs to the CYP111 family of heme-dependent cytochrome P450 monooxygenases Novosphingobium aromaticivorans