BRENDA - Enzyme Database

A cytochrome P450 class I electron transfer system from Novosphingobium aromaticivorans

Bell, S.G.; Dale, A.; Rees, N.H.; Wong, L.L.; Appl. Microbiol. Biotechnol. 86, 163-175 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.14.84
CYP111A2 cloning, expression in Escherichia coli strain DH5alpha
Novosphingobium aromaticivorans
1.14.15.1
ArR/Arx electron-transport chain co-expressed with the CYP enzymes in Escherichia coli. Amplified genes incorporated into the pRSFDuet-Arx vector using the Nde I and Kpn I/EcoR V restriction sites, plasmids pETDuet-Arx-ArR and one of the P450 expressing plasmids pRSFDuet-Arx-CYP transformed into Escherichia coli competent BL21(DE3) cells
Novosphingobium aromaticivorans
Engineering
EC Number
Protein Variants
Commentary
Organism
1.14.14.84
additional information
interaction and coupling of CYP enzyme reactions from Novosphingobium aromaticivorans with ferredoxin reductases ArR from Novosphingobium aromaticivorans and PdR from from Rhodopseudomonas palustris CGA009, activity reconstitution with the electron transfer system of ArR and Arx, overview
Novosphingobium aromaticivorans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.14.84
Fe2+
in the heme cofactor
Novosphingobium aromaticivorans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.14.84
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
Novosphingobium aromaticivorans
-
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
?
1.14.14.84
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
Novosphingobium aromaticivorans ATCC 700278D-5
-
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.14.84
Novosphingobium aromaticivorans
-
-
-
1.14.14.84
Novosphingobium aromaticivorans ATCC 700278D-5
-
-
-
1.14.15.1
Novosphingobium aromaticivorans
-
strain ATCC 700278D-5
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.14.14.84
recombinant enzyme from Escherichia coli by ultrafiltration and anion exchange chromatography
Novosphingobium aromaticivorans
Storage Stability
EC Number
Storage Stability
Organism
1.14.15.1
-20°C, 50 mM Tris buffer, pH 7.4, 50% v/v glycerol
Novosphingobium aromaticivorans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.14.84
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
701873
Novosphingobium aromaticivorans
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
-
?
1.14.14.84
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
701873
Novosphingobium aromaticivorans
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
NMR spectroscopy product analysis
-
-
?
1.14.14.84
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
701873
Novosphingobium aromaticivorans ATCC 700278D-5
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
-
?
1.14.15.1
(+)-camphor + O2 + reduced putidaredoxin
CYP101D1, CYP101D2
701873
Novosphingobium aromaticivorans
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
-
-
?
1.14.15.1
(+)-camphor + O2 + reduced putidaredoxin
CYP101D1, CYP101D2
701873
Novosphingobium aromaticivorans ATCC 700278D-5
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
-
-
?
1.14.15.1
2-adamantanone + O2 + reduced putidaredoxin
CYP101D1 and CYP101D2
701873
Novosphingobium aromaticivorans
5-hydroxy-2-adamantanone + oxidized putidaredoxin + H2O
-
-
-
?
1.14.15.1
2-adamantanone + O2 + reduced putidaredoxin
CYP101D1 and CYP101D2
701873
Novosphingobium aromaticivorans ATCC 700278D-5
5-hydroxy-2-adamantanone + oxidized putidaredoxin + H2O
-
-
-
?
1.14.15.1
beta-ionone + O2 + reduced putidaredoxin
CYP101C1 and CYP101B1. CYP101C1 oxidizes beta-ionone to 4-hydroxy-beta-ionone (75%) with one other, unidentified product. This latter compound is the major product of beta-ionone oxidation by CYP101B1 (90%) where 4-hydroxy-beta-ionone is the minor product (10%)
701873
Novosphingobium aromaticivorans
4-hydroxy-beta-ionone + oxidized putidaredoxin + H2O
-
-
-
?
1.14.15.1
beta-ionone + O2 + reduced putidaredoxin
CYP101C1 and CYP101B1. CYP101C1 oxidizes beta-ionone to 4-hydroxy-beta-ionone (75%) with one other, unidentified product. This latter compound is the major product of beta-ionone oxidation by CYP101B1 (90%) where 4-hydroxy-beta-ionone is the minor product (10%)
701873
Novosphingobium aromaticivorans ATCC 700278D-5
4-hydroxy-beta-ionone + oxidized putidaredoxin + H2O
-
-
-
?
1.14.15.1
linalool + O2 + reduced putidaredoxin
CYP111A2 and CYP111A1
701873
Novosphingobium aromaticivorans
8-hydroxy-linalool + oxidized putidaredoxin + H2O
-
-
-
?
1.14.15.1
linalool + O2 + reduced putidaredoxin
CYP111A2 and CYP111A1
701873
Novosphingobium aromaticivorans ATCC 700278D-5
8-hydroxy-linalool + oxidized putidaredoxin + H2O
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.14.84
CYP111A2
-
Novosphingobium aromaticivorans
1.14.14.84
linalool hydroxylase
-
Novosphingobium aromaticivorans
1.14.15.1
CYP101B1
-
Novosphingobium aromaticivorans
1.14.15.1
CYP101C1
-
Novosphingobium aromaticivorans
1.14.15.1
CYP101D1
-
Novosphingobium aromaticivorans
1.14.15.1
CYP101D2
-
Novosphingobium aromaticivorans
1.14.15.1
CYP111A2
-
Novosphingobium aromaticivorans
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.14.84
30
-
assay at
Novosphingobium aromaticivorans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.84
7.4
-
assay at
Novosphingobium aromaticivorans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.84
cytochrome P450
-
Novosphingobium aromaticivorans
1.14.14.84
heme
dependent on
Novosphingobium aromaticivorans
1.14.15.1
putidaredoxin
-
Novosphingobium aromaticivorans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.84
CYP111A2 cloning, expression in Escherichia coli strain DH5alpha
Novosphingobium aromaticivorans
1.14.15.1
ArR/Arx electron-transport chain co-expressed with the CYP enzymes in Escherichia coli. Amplified genes incorporated into the pRSFDuet-Arx vector using the Nde I and Kpn I/EcoR V restriction sites, plasmids pETDuet-Arx-ArR and one of the P450 expressing plasmids pRSFDuet-Arx-CYP transformed into Escherichia coli competent BL21(DE3) cells
Novosphingobium aromaticivorans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.84
cytochrome P450
-
Novosphingobium aromaticivorans
1.14.14.84
heme
dependent on
Novosphingobium aromaticivorans
1.14.15.1
putidaredoxin
-
Novosphingobium aromaticivorans
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
1.14.14.84
additional information
interaction and coupling of CYP enzyme reactions from Novosphingobium aromaticivorans with ferredoxin reductases ArR from Novosphingobium aromaticivorans and PdR from from Rhodopseudomonas palustris CGA009, activity reconstitution with the electron transfer system of ArR and Arx, overview
Novosphingobium aromaticivorans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.14.84
Fe2+
in the heme cofactor
Novosphingobium aromaticivorans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.14.84
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
Novosphingobium aromaticivorans
-
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
?
1.14.14.84
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
Novosphingobium aromaticivorans ATCC 700278D-5
-
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.84
recombinant enzyme from Escherichia coli by ultrafiltration and anion exchange chromatography
Novosphingobium aromaticivorans
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.15.1
-20°C, 50 mM Tris buffer, pH 7.4, 50% v/v glycerol
Novosphingobium aromaticivorans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.14.84
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
701873
Novosphingobium aromaticivorans
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
-
?
1.14.14.84
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
701873
Novosphingobium aromaticivorans
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
NMR spectroscopy product analysis
-
-
?
1.14.14.84
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
701873
Novosphingobium aromaticivorans ATCC 700278D-5
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
-
?
1.14.15.1
(+)-camphor + O2 + reduced putidaredoxin
CYP101D1, CYP101D2
701873
Novosphingobium aromaticivorans
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
-
-
?
1.14.15.1
(+)-camphor + O2 + reduced putidaredoxin
CYP101D1, CYP101D2
701873
Novosphingobium aromaticivorans ATCC 700278D-5
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
-
-
?
1.14.15.1
2-adamantanone + O2 + reduced putidaredoxin
CYP101D1 and CYP101D2
701873
Novosphingobium aromaticivorans
5-hydroxy-2-adamantanone + oxidized putidaredoxin + H2O
-
-
-
?
1.14.15.1
2-adamantanone + O2 + reduced putidaredoxin
CYP101D1 and CYP101D2
701873
Novosphingobium aromaticivorans ATCC 700278D-5
5-hydroxy-2-adamantanone + oxidized putidaredoxin + H2O
-
-
-
?
1.14.15.1
beta-ionone + O2 + reduced putidaredoxin
CYP101C1 and CYP101B1. CYP101C1 oxidizes beta-ionone to 4-hydroxy-beta-ionone (75%) with one other, unidentified product. This latter compound is the major product of beta-ionone oxidation by CYP101B1 (90%) where 4-hydroxy-beta-ionone is the minor product (10%)
701873
Novosphingobium aromaticivorans
4-hydroxy-beta-ionone + oxidized putidaredoxin + H2O
-
-
-
?
1.14.15.1
beta-ionone + O2 + reduced putidaredoxin
CYP101C1 and CYP101B1. CYP101C1 oxidizes beta-ionone to 4-hydroxy-beta-ionone (75%) with one other, unidentified product. This latter compound is the major product of beta-ionone oxidation by CYP101B1 (90%) where 4-hydroxy-beta-ionone is the minor product (10%)
701873
Novosphingobium aromaticivorans ATCC 700278D-5
4-hydroxy-beta-ionone + oxidized putidaredoxin + H2O
-
-
-
?
1.14.15.1
linalool + O2 + reduced putidaredoxin
CYP111A2 and CYP111A1
701873
Novosphingobium aromaticivorans
8-hydroxy-linalool + oxidized putidaredoxin + H2O
-
-
-
?
1.14.15.1
linalool + O2 + reduced putidaredoxin
CYP111A2 and CYP111A1
701873
Novosphingobium aromaticivorans ATCC 700278D-5
8-hydroxy-linalool + oxidized putidaredoxin + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.14.84
30
-
assay at
Novosphingobium aromaticivorans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.84
7.4
-
assay at
Novosphingobium aromaticivorans
General Information
EC Number
General Information
Commentary
Organism
1.14.14.84
evolution
the enzyme belongs to the CYP111 family of heme-dependent cytochrome P450 monooxygenases
Novosphingobium aromaticivorans
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.14.84
evolution
the enzyme belongs to the CYP111 family of heme-dependent cytochrome P450 monooxygenases
Novosphingobium aromaticivorans