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Literature summary extracted from

  • Park, A.R.; Oh, D.K.
    Galacto-oligosaccharide production using microbial beta-galactosidase: current state and perspectives (2010), Appl. Microbiol. Biotechnol., 85, 1279-1286.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 D-galactose uncompetitive inhibition Caldicellulosiruptor saccharolyticus
3.2.1.23 D-galactose competitive inhibition Kluyveromyces lactis
3.2.1.23 D-galactose competitive inhibition Limosilactobacillus reuteri
3.2.1.23 D-galactose competitive inhibition Saccharopolyspora rectivirgula
3.2.1.23 D-galactose competitive inhibition Thermus sp.
3.2.1.23 D-glucose uncompetitive inhibition Caldicellulosiruptor saccharolyticus
3.2.1.23 D-glucose noncompetitive inhibition Kluyveromyces lactis
3.2.1.23 D-glucose competitive inhibition Limosilactobacillus reuteri
3.2.1.23 D-glucose competitive inhibition Saccharolobus solfataricus
3.2.1.23 D-glucose noncompetitive inhibition Saccharopolyspora rectivirgula
3.2.1.23 D-glucose noncompetitive inhibition using 4-nitrophenyl-beta-D-galactopyranoside as substrate and uncompetitive inhibition using lactose as substrate Thermus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.1
-
 4-nitrophenyl beta-D-galactopyranoside
-
 Saccharopolyspora rectivirgula
3.2.1.23 0.1
-
 4-nitrophenyl-beta-D-galactopyranoside
-
 Caldicellulosiruptor saccharolyticus
3.2.1.23 0.2
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Saccharolobus solfataricus
3.2.1.23 0.7
-
 lactose
-
 Saccharopolyspora rectivirgula
3.2.1.23 0.7
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Lactobacillus acidophilus
3.2.1.23 1
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Limosilactobacillus reuteri
3.2.1.23 1.2
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Caldicellulosiruptor saccharolyticus
3.2.1.23 2.7
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Arthrobacter psychrolactophilus
3.2.1.23 3.3
-
 4-nitrophenyl-beta-D-galactopyranoside
-
 Aspergillus aculeatus
3.2.1.23 4
-
 lactose
-
 Lactobacillus acidophilus
3.2.1.23 13
-
 lactose
-
 Saccharolobus solfataricus
3.2.1.23 30
-
 lactose
-
 Caldicellulosiruptor saccharolyticus
3.2.1.23 31
-
 lactose
-
 Limosilactobacillus reuteri
3.2.1.23 35
-
 lactose
-
 Aspergillus aculeatus
3.2.1.23 42
-
 lactose
-
 Arthrobacter psychrolactophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Alicyclobacillus acidocaldarius
-
-
-
3.2.1.23 Arthrobacter psychrolactophilus
-
-
-
3.2.1.23 Aspergillus aculeatus
-
-
-
3.2.1.23 Aspergillus oryzae
-
-
-
3.2.1.23 Bifidobacterium adolescentis
-
-
-
3.2.1.23 Bifidobacterium bifidum
-
-
-
3.2.1.23 Bifidobacterium longum subsp. infantis
-
-
-
3.2.1.23 Caldicellulosiruptor saccharolyticus
-
-
-
3.2.1.23 Geobacillus stearothermophilus
-
-
-
3.2.1.23 Hamamotoa singularis
-
-
-
3.2.1.23 Kluyveromyces lactis
-
-
-
3.2.1.23 Kluyveromyces marxianus
-
-
-
3.2.1.23 Lactobacillus acidophilus
-
-
-
3.2.1.23 Limosilactobacillus reuteri
-
-
-
3.2.1.23 Niallia circulans
-
-
-
3.2.1.23 Pantoea agglomerans
-
-
-
3.2.1.23 Papiliotrema laurentii
-
-
-
3.2.1.23 Priestia megaterium
-
-
-
3.2.1.23 Saccharolobus solfataricus
-
-
-
3.2.1.23 Saccharopolyspora rectivirgula
-
-
-
3.2.1.23 Sterigmatomyces elviae
-
-
-
3.2.1.23 Streptococcus pneumoniae
-
-
-
3.2.1.23 Thermotoga maritima
-
-
-
3.2.1.23 Thermus aquaticus
-
-
-
3.2.1.23 Thermus sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermus aquaticus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Geobacillus stearothermophilus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Priestia megaterium 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Streptococcus pneumoniae 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Pantoea agglomerans 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Niallia circulans 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bifidobacterium bifidum 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Kluyveromyces marxianus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bifidobacterium adolescentis 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Aspergillus oryzae 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermus sp. 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Papiliotrema laurentii 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Lactobacillus acidophilus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Caldicellulosiruptor saccharolyticus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermotoga maritima 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Limosilactobacillus reuteri 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bifidobacterium longum subsp. infantis 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Kluyveromyces lactis 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Aspergillus aculeatus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Alicyclobacillus acidocaldarius 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Hamamotoa singularis 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharopolyspora rectivirgula 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Sterigmatomyces elviae 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Arthrobacter psychrolactophilus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermus aquaticus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Geobacillus stearothermophilus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Priestia megaterium 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Streptococcus pneumoniae 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Pantoea agglomerans 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Niallia circulans 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bifidobacterium bifidum 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Kluyveromyces marxianus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bifidobacterium adolescentis 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Aspergillus oryzae 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermus sp. 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Papiliotrema laurentii 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Lactobacillus acidophilus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Caldicellulosiruptor saccharolyticus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermotoga maritima 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Limosilactobacillus reuteri 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bifidobacterium longum subsp. infantis 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Aspergillus aculeatus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Alicyclobacillus acidocaldarius 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Hamamotoa singularis 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharopolyspora rectivirgula 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Sterigmatomyces elviae 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Thermus aquaticus D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Geobacillus stearothermophilus D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Priestia megaterium D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Streptococcus pneumoniae D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Pantoea agglomerans D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Niallia circulans D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Bifidobacterium bifidum D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Kluyveromyces marxianus D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Bifidobacterium adolescentis D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Saccharolobus solfataricus D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Aspergillus oryzae D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Thermus sp. D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Papiliotrema laurentii D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Lactobacillus acidophilus D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Caldicellulosiruptor saccharolyticus D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Thermotoga maritima D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Limosilactobacillus reuteri D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Bifidobacterium longum subsp. infantis D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Kluyveromyces lactis D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Aspergillus aculeatus D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Alicyclobacillus acidocaldarius D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Hamamotoa singularis D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Saccharopolyspora rectivirgula D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Sterigmatomyces elviae D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Arthrobacter psychrolactophilus D-galactose + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Thermus aquaticus
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Geobacillus stearothermophilus
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Priestia megaterium
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Streptococcus pneumoniae
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Pantoea agglomerans
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Niallia circulans
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Bifidobacterium bifidum
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Kluyveromyces marxianus
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Bifidobacterium adolescentis
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Saccharolobus solfataricus
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Aspergillus oryzae
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Thermus sp.
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Papiliotrema laurentii
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Lactobacillus acidophilus
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Caldicellulosiruptor saccharolyticus
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Thermotoga maritima
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Limosilactobacillus reuteri
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Bifidobacterium longum subsp. infantis
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Kluyveromyces lactis
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Aspergillus aculeatus
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Alicyclobacillus acidocaldarius
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Hamamotoa singularis
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Saccharopolyspora rectivirgula
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Sterigmatomyces elviae
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Arthrobacter psychrolactophilus
3.2.1.23 lactase
-
 Thermus aquaticus
3.2.1.23 lactase
-
 Geobacillus stearothermophilus
3.2.1.23 lactase
-
 Priestia megaterium
3.2.1.23 lactase
-
 Streptococcus pneumoniae
3.2.1.23 lactase
-
 Pantoea agglomerans
3.2.1.23 lactase
-
 Niallia circulans
3.2.1.23 lactase
-
 Bifidobacterium bifidum
3.2.1.23 lactase
-
 Kluyveromyces marxianus
3.2.1.23 lactase
-
 Bifidobacterium adolescentis
3.2.1.23 lactase
-
 Saccharolobus solfataricus
3.2.1.23 lactase
-
 Aspergillus oryzae
3.2.1.23 lactase
-
 Thermus sp.
3.2.1.23 lactase
-
 Papiliotrema laurentii
3.2.1.23 lactase
-
 Lactobacillus acidophilus
3.2.1.23 lactase
-
 Caldicellulosiruptor saccharolyticus
3.2.1.23 lactase
-
 Thermotoga maritima
3.2.1.23 lactase
-
 Limosilactobacillus reuteri
3.2.1.23 lactase
-
 Bifidobacterium longum subsp. infantis
3.2.1.23 lactase
-
 Kluyveromyces lactis
3.2.1.23 lactase
-
 Aspergillus aculeatus
3.2.1.23 lactase
-
 Alicyclobacillus acidocaldarius
3.2.1.23 lactase
-
 Hamamotoa singularis
3.2.1.23 lactase
-
 Saccharopolyspora rectivirgula
3.2.1.23 lactase
-
 Sterigmatomyces elviae
3.2.1.23 lactase
-
 Arthrobacter psychrolactophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 10
-
-
 Arthrobacter psychrolactophilus
3.2.1.23 30
-
-
 Streptococcus pneumoniae
3.2.1.23 30
-
-
 Kluyveromyces marxianus
3.2.1.23 30
-
-
 Aspergillus oryzae
3.2.1.23 30
-
-
 Hamamotoa singularis
3.2.1.23 37
-
-
 Bifidobacterium bifidum
3.2.1.23 37 40
-
 Pantoea agglomerans
3.2.1.23 37 40
-
 Kluyveromyces lactis
3.2.1.23 40
-
-
 Priestia megaterium
3.2.1.23 50
-
-
 Niallia circulans
3.2.1.23 50
-
-
 Bifidobacterium adolescentis
3.2.1.23 50
-
-
 Limosilactobacillus reuteri
3.2.1.23 50
-
-
 Hamamotoa singularis
3.2.1.23 50 60
-
 Bifidobacterium longum subsp. infantis
3.2.1.23 55
-
-
 Lactobacillus acidophilus
3.2.1.23 55 60
-
 Aspergillus aculeatus
3.2.1.23 58
-
-
 Papiliotrema laurentii
3.2.1.23 60
-
-
 Saccharopolyspora rectivirgula
3.2.1.23 65
-
-
 Geobacillus stearothermophilus
3.2.1.23 65
-
-
 Alicyclobacillus acidocaldarius
3.2.1.23 70
-
-
 Geobacillus stearothermophilus
3.2.1.23 70
-
-
 Thermus sp.
3.2.1.23 75
-
-
 Saccharolobus solfataricus
3.2.1.23 80
-
-
 Thermus aquaticus
3.2.1.23 80
-
-
 Caldicellulosiruptor saccharolyticus
3.2.1.23 80 85
-
 Thermotoga maritima
3.2.1.23 85
-
-
 Sterigmatomyces elviae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 30
-
 the enzyme shows a half-life of 60 min at 30°C Arthrobacter psychrolactophilus
3.2.1.23 37
-
 the enzyme shows a half-life of 2880 min at 37°C Lactobacillus acidophilus
3.2.1.23 40
-
 the enzyme shows a half-life of 300 min at 40°C Kluyveromyces marxianus
3.2.1.23 45
-
 the enzyme shows a half-life of 120 min at 45°C Hamamotoa singularis
3.2.1.23 45
-
 the enzyme shows a half-life of 240 min at 45°C Bifidobacterium bifidum
3.2.1.23 50
-
 the enzyme shows a half-life of 10 min at 50°C Bifidobacterium adolescentis
3.2.1.23 58
-
 the enzyme shows a half-life of 10 min at 58°C Papiliotrema laurentii
3.2.1.23 60
-
 the enzyme shows a half-life of 120 min at 60°C Bifidobacterium longum subsp. infantis
3.2.1.23 60
-
 the enzyme shows a half-life of 240 min at 60°C Saccharopolyspora rectivirgula
3.2.1.23 70
-
 the enzyme shows a half-life of 10 min at 70°C Aspergillus oryzae
3.2.1.23 70
-
 the enzyme shows a half-life of 1200 min at 70°C Thermus sp.
3.2.1.23 70
-
 the enzyme shows a half-life of 2880 min at 70°C Caldicellulosiruptor saccharolyticus
3.2.1.23 70
-
 the enzyme shows a half-life of 540 min at 70°C Geobacillus stearothermophilus
3.2.1.23 80
-
 the enzyme shows a half-life of 6 min at 80°C Alicyclobacillus acidocaldarius
3.2.1.23 80
-
 the enzyme shows a half-life of 60 min at 80°C Sterigmatomyces elviae
3.2.1.23 85
-
 the enzyme shows a half-life of 180 min at 85°C Saccharolobus solfataricus
3.2.1.23 90
-
 the enzyme shows a half-life of 16 min at 90°C Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 1.9
-
 lactose
-
 Saccharolobus solfataricus
3.2.1.23 3
-
 lactose
-
 Arthrobacter psychrolactophilus
3.2.1.23 13
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Arthrobacter psychrolactophilus
3.2.1.23 32
-
 4-nitrophenyl-beta-D-galactopyranoside
-
 Caldicellulosiruptor saccharolyticus
3.2.1.23 42
-
 lactose
-
 Caldicellulosiruptor saccharolyticus
3.2.1.23 50
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Lactobacillus acidophilus
3.2.1.23 56
-
 4-nitrophenyl beta-D-galactopyranoside
-
 Saccharopolyspora rectivirgula
3.2.1.23 58
-
 lactose
-
 Limosilactobacillus reuteri
3.2.1.23 63
-
 lactose
-
 Saccharopolyspora rectivirgula
3.2.1.23 95
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Saccharolobus solfataricus
3.2.1.23 149
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Caldicellulosiruptor saccharolyticus
3.2.1.23 254
-
 lactose
-
 Aspergillus aculeatus
3.2.1.23 338
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Limosilactobacillus reuteri
3.2.1.23 632
-
 lactose
-
 Lactobacillus acidophilus
3.2.1.23 932
-
 4-nitrophenyl-beta-D-galactopyranoside
-
 Aspergillus aculeatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 4
-
-
 Hamamotoa singularis
3.2.1.23 4.3
-
-
 Papiliotrema laurentii
3.2.1.23 4.5 5
-
 Sterigmatomyces elviae
3.2.1.23 4.8
-
-
 Aspergillus oryzae
3.2.1.23 5
-
-
 Hamamotoa singularis
3.2.1.23 5 6
-
 Niallia circulans
3.2.1.23 5.4
-
-
 Aspergillus aculeatus
3.2.1.23 5.5
-
-
 Thermus aquaticus
3.2.1.23 5.5
-
-
 Alicyclobacillus acidocaldarius
3.2.1.23 5.5 7.5
-
 Streptococcus pneumoniae
3.2.1.23 6 9
-
 Priestia megaterium
3.2.1.23 6
-
-
 Bifidobacterium adolescentis
3.2.1.23 6
-
-
 Caldicellulosiruptor saccharolyticus
3.2.1.23 6 8
-
 Limosilactobacillus reuteri
3.2.1.23 6.5
-
-
 Geobacillus stearothermophilus
3.2.1.23 6.5
-
-
 Bifidobacterium bifidum
3.2.1.23 6.5
-
-
 Kluyveromyces marxianus
3.2.1.23 6.5
-
-
 Saccharolobus solfataricus
3.2.1.23 6.5
-
-
 Thermus sp.
3.2.1.23 6.5
-
-
 Thermotoga maritima
3.2.1.23 6.5 8
-
 Lactobacillus acidophilus
3.2.1.23 6.5 7.2
-
 Saccharopolyspora rectivirgula
3.2.1.23 6.6 7
-
 Kluyveromyces lactis
3.2.1.23 7
-
-
 Geobacillus stearothermophilus
3.2.1.23 7.5
-
-
 Bifidobacterium longum subsp. infantis
3.2.1.23 7.5 8
-
 Pantoea agglomerans
3.2.1.23 8
-
-
 Arthrobacter psychrolactophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.23 1.17
-
 D-glucose at 80°C and pH 6.0 Caldicellulosiruptor saccharolyticus
3.2.1.23 3
-
 D-galactose at 70°C and pH 6.5, using 4-nitrophenyl-beta-D-galactopyranoside as substrate Thermus sp.
3.2.1.23 12
-
 D-galactose at 80°C and pH 6.0 Caldicellulosiruptor saccharolyticus
3.2.1.23 45
-
 D-galactose at 25°C and pH 6.5, using 2-nitrophenyl-beta-D-galactopyranoside as substrate Kluyveromyces lactis
3.2.1.23 50
-
 D-glucose at 70°C and pH 6.5, using 4-nitrophenyl-beta-D-galactopyranoside as substrate Thermus sp.
3.2.1.23 89
-
 D-galactose at 30°C and pH 6.5, using lactose as substrate Limosilactobacillus reuteri
3.2.1.23 90
-
 D-galactose at 37°C and pH 7.0, using lactose as substrate Kluyveromyces lactis
3.2.1.23 96
-
 D-glucose at 75°C and pH 6.5 Saccharolobus solfataricus
3.2.1.23 115
-
 D-galactose at 30°C and pH 6.5, using 2-nitrophenyl-beta-D-galactopyranoside as substrate Limosilactobacillus reuteri
3.2.1.23 683
-
 D-glucose at 30°C and pH 6.5, using 2-nitrophenyl-beta-D-galactopyranoside as substrate Limosilactobacillus reuteri
3.2.1.23 758
-
 D-glucose at 25°C and pH 6.5, using 2-nitrophenyl-beta-D-galactopyranoside as substrate Kluyveromyces lactis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.23 0.1
-
 lactose
-
 Arthrobacter psychrolactophilus
3.2.1.23 0.3
-
 lactose
-
 Saccharolobus solfataricus
3.2.1.23 1.4
-
 lactose
-
 Caldicellulosiruptor saccharolyticus
3.2.1.23 1.9
-
 lactose
-
 Limosilactobacillus reuteri
3.2.1.23 4.7
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Arthrobacter psychrolactophilus
3.2.1.23 7
-
 lactose
-
 Aspergillus aculeatus
3.2.1.23 13
-
 lactose
-
 Lactobacillus acidophilus
3.2.1.23 47
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Saccharolobus solfataricus
3.2.1.23 84
-
 lactose
-
 Saccharopolyspora rectivirgula
3.2.1.23 123
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Caldicellulosiruptor saccharolyticus
3.2.1.23 284
-
 4-nitrophenyl-beta-D-galactopyranoside
-
 Aspergillus aculeatus
3.2.1.23 296
-
 4-nitrophenyl-beta-D-galactopyranoside
-
 Caldicellulosiruptor saccharolyticus
3.2.1.23 345
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Limosilactobacillus reuteri
3.2.1.23 865
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Lactobacillus acidophilus
3.2.1.23 1395
-
 4-nitrophenyl-beta-D-galactopyranoside
-
 Saccharopolyspora rectivirgula