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Literature summary extracted from
- Enhanced levan production using chitin-binding domain fused levansucrase immobilized on chitin beads (2009), Appl. Microbiol. Biotechnol., 82, 445-451.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 2.4.1.10 | synthesis | the enzyme is useful in levan production for application in the food and pharmaceutical industry | Zymomonas mobilis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.10 | gene levU, overexpression of the His-tagged-LevU and of enzyme fused to a chitin-binding domain in Escherichia coli strain DH5alpha | Zymomonas mobilis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 2.4.1.10 | additional information | enhanced levan production using chitin-binding domain fused levansucrase immobilized on chitin beads | Zymomonas mobilis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.10 | sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside | Zymomonas mobilis | - |
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.10 | Zymomonas mobilis | - |
strain ATCC 10988, gene levU | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.10 | recombinant His-tagged LevU by metal affinity chromatography, recombinant LevU fused to a chitin-binding domain from Escherichia coli strain DH5alpha by affinity binding and immobilization on chitin beads | Zymomonas mobilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.10 | sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside | - |
Zymomonas mobilis | D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.10 | 5 | 15 | - |
Zymomonas mobilis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.10 | 5 | 30 | inactive above | Zymomonas mobilis |
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Release 2023.1 (January 2023)
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