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Literature summary extracted from

  • Brat, D.; Boles, E.; Wiedemann, B.
    Functional expression of a bacterial xylose isomerase in Saccharomyces cerevisiae (2009), Appl. Environ. Microbiol., 75, 2304-2311.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.5 phylogenetic analysis and enzyme sequence comparisons, functional expression of codon-optimized enzyme in Saccharomyces cerevisiae confers on the yeast cells the ability to metabolize D-xylose and to use it as the sole carbon and energy source. The recombinant enzyme shows reduced sensitivity to inhibition by xylitol Piromyces sp.
5.3.1.5 phylogenetic analysis and enzyme sequence comparisons, functional expression of codon-optimized enzyme in Saccharomyces cerevisiae confers on the yeast cells the ability to metabolize D-xylose and to use it as the sole carbon and energy source. The recombinant enzyme shows reduced sensitivity to inhibition by xylitol Lachnoclostridium phytofermentans

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.5 xylitol
-
 Lachnoclostridium phytofermentans
5.3.1.5 xylitol
-
 Piromyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 49.9
-
 D-xylose pH 7.5, recombinant enzyme Piromyces sp.
5.3.1.5 61.9
-
 D-xylose pH 7.5, native enzyme Lachnoclostridium phytofermentans
5.3.1.5 66
-
 D-xylose pH 7.5, recombinant enzyme Lachnoclostridium phytofermentans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.5 Mg2+ required Piromyces sp.
5.3.1.5 Mg2+ required Lachnoclostridium phytofermentans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.5 D-Xylose Piromyces sp.
-
 D-Xylulose
-
 ?
5.3.1.5 D-Xylose Lachnoclostridium phytofermentans
-
 D-Xylulose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Lachnoclostridium phytofermentans
-
-
-
5.3.1.5 Piromyces sp.
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.5 0.0076
-
 native enzyme in cell extract Lachnoclostridium phytofermentans
5.3.1.5 0.0344
-
 recombinant enzyme in yeast cell extract Lachnoclostridium phytofermentans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Xylose
-
 Piromyces sp. D-Xylulose
-
 ?
5.3.1.5 D-Xylose
-
 Lachnoclostridium phytofermentans D-Xylulose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 7.5
-
 assay at Piromyces sp.
5.3.1.5 7.5
-
 assay at Lachnoclostridium phytofermentans