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Literature summary extracted from

  • Prakash, O.; Jaiswal, N.
    alpha-Amylase: an ideal representative of thermostable enzymes (2010), Appl. Biochem. Biotechnol., 160, 2401-2414.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.1 N172R/H156Y/A181T the mutations increase the thermostability of alpha-amylase by 5fold Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Ca2+ Bacillus subtilis alpha-amylase requires roughly 4times more calcium for full activity than other alpha-amylases of nonbacterial nature Bacillus subtilis
3.2.1.1 additional information does not require Ca2+ Pyrococcus furiosus
3.2.1.1 additional information does not require Ca2+ Pyrococcus woesei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 58000
-
-
Bacillus licheniformis
3.2.1.1 58000
-
SDS-PAGE Bacillus amyloliquefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.1 glycogen + H2O Lactiplantibacillus plantarum
-
?
-
?
3.2.1.1 glycogen + H2O Bacillus subtilis
-
?
-
?
3.2.1.1 glycogen + H2O Geobacillus stearothermophilus
-
?
-
?
3.2.1.1 glycogen + H2O Pyrococcus furiosus
-
?
-
?
3.2.1.1 glycogen + H2O Bacillus licheniformis
-
?
-
?
3.2.1.1 glycogen + H2O Bacillus amyloliquefaciens
-
?
-
?
3.2.1.1 glycogen + H2O Rhodothermus marinus
-
?
-
?
3.2.1.1 glycogen + H2O Pyrococcus woesei
-
?
-
?
3.2.1.1 glycogen + H2O Thermotoga maritima
-
?
-
?
3.2.1.1 glycogen + H2O Lederbergia lentus
-
?
-
?
3.2.1.1 glycogen + H2O Thermoactinomyces vulgaris
-
?
-
?
3.2.1.1 glycogen + H2O Rhizopus sp.
-
?
-
?
3.2.1.1 glycogen + H2O Thermomyces lanuginosus
-
?
-
?
3.2.1.1 glycogen + H2O Thermococcus litoralis
-
?
-
?
3.2.1.1 glycogen + H2O Thermothelomyces heterothallicus
-
?
-
?
3.2.1.1 glycogen + H2O Chloroflexus aurantiacus
-
?
-
?
3.2.1.1 glycogen + H2O Thermus filiformis
-
?
-
?
3.2.1.1 glycogen + H2O Mycothermus thermophilus
-
?
-
?
3.2.1.1 glycogen + H2O Lipomyces kononenkoae
-
?
-
?
3.2.1.1 glycogen + H2O Alicyclobacillus acidocaldarius
-
?
-
?
3.2.1.1 glycogen + H2O Dictyoglomus thermophilum
-
?
-
?
3.2.1.1 glycogen + H2O Thermococcus hydrothermalis
-
?
-
?
3.2.1.1 glycogen + H2O Desulfurococcus mucosus
-
?
-
?
3.2.1.1 glycogen + H2O Anoxybacillus flavithermus
-
?
-
?
3.2.1.1 glycogen + H2O Thermococcus profundus
-
?
-
?
3.2.1.1 glycogen + H2O Pyrodictium abyssi
-
?
-
?
3.2.1.1 glycogen + H2O Thermococcus fumicolans
-
?
-
?
3.2.1.1 glycogen + H2O Staphylothermus marinus
-
?
-
?
3.2.1.1 glycogen + H2O Lactobacillus amylovorus
-
?
-
?
3.2.1.1 glycogen + H2O Halothermothrix orenii
-
?
-
?
3.2.1.1 glycogen + H2O Thermococcus aggregans
-
?
-
?
3.2.1.1 glycogen + H2O Thermococcus celer
-
?
-
?
3.2.1.1 glycogen + H2O Thermococcus guaymasensis
-
?
-
?
3.2.1.1 starch + H2O Lactiplantibacillus plantarum
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Bacillus subtilis
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Geobacillus stearothermophilus
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Pyrococcus furiosus
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Bacillus licheniformis
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Bacillus amyloliquefaciens
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Rhodothermus marinus
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Pyrococcus woesei
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Thermotoga maritima
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Lederbergia lentus
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Thermoactinomyces vulgaris
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Rhizopus sp.
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Thermomyces lanuginosus
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Thermococcus litoralis
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Thermothelomyces heterothallicus
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Chloroflexus aurantiacus
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Thermus filiformis
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Mycothermus thermophilus
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Lipomyces kononenkoae
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Alicyclobacillus acidocaldarius
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Dictyoglomus thermophilum
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Thermococcus hydrothermalis
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Desulfurococcus mucosus
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Anoxybacillus flavithermus
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Thermococcus profundus
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Pyrodictium abyssi
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Thermococcus fumicolans
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Staphylothermus marinus
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Lactobacillus amylovorus
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Halothermothrix orenii
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Thermococcus aggregans
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Thermococcus celer
-
malto-oligosaccharides
-
?
3.2.1.1 starch + H2O Thermococcus guaymasensis
-
malto-oligosaccharides
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Alicyclobacillus acidocaldarius
-
-
-
3.2.1.1 Anoxybacillus flavithermus
-
-
-
3.2.1.1 Bacillus amyloliquefaciens
-
-
-
3.2.1.1 Bacillus licheniformis
-
-
-
3.2.1.1 Bacillus subtilis
-
-
-
3.2.1.1 Chloroflexus aurantiacus
-
-
-
3.2.1.1 Desulfurococcus mucosus
-
-
-
3.2.1.1 Dictyoglomus thermophilum
-
-
-
3.2.1.1 Geobacillus stearothermophilus
-
-
-
3.2.1.1 Halothermothrix orenii
-
-
-
3.2.1.1 Lactiplantibacillus plantarum
-
-
-
3.2.1.1 Lactobacillus amylovorus
-
-
-
3.2.1.1 Lederbergia lentus
-
-
-
3.2.1.1 Lipomyces kononenkoae
-
-
-
3.2.1.1 Mycothermus thermophilus
-
-
-
3.2.1.1 Pyrococcus furiosus
-
-
-
3.2.1.1 Pyrococcus woesei
-
-
-
3.2.1.1 Pyrodictium abyssi
-
-
-
3.2.1.1 Rhizopus sp.
-
-
-
3.2.1.1 Rhodothermus marinus
-
-
-
3.2.1.1 Staphylothermus marinus
-
-
-
3.2.1.1 Thermoactinomyces vulgaris
-
-
-
3.2.1.1 Thermococcus aggregans
-
-
-
3.2.1.1 Thermococcus celer
-
-
-
3.2.1.1 Thermococcus fumicolans
-
-
-
3.2.1.1 Thermococcus guaymasensis
-
-
-
3.2.1.1 Thermococcus hydrothermalis
-
-
-
3.2.1.1 Thermococcus litoralis
-
-
-
3.2.1.1 Thermococcus profundus
-
-
-
3.2.1.1 Thermomyces lanuginosus
-
-
-
3.2.1.1 Thermothelomyces heterothallicus
-
-
-
3.2.1.1 Thermotoga maritima
-
-
-
3.2.1.1 Thermus filiformis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 glycogen + H2O
-
Lactiplantibacillus plantarum ?
-
?
3.2.1.1 glycogen + H2O
-
Bacillus subtilis ?
-
?
3.2.1.1 glycogen + H2O
-
Geobacillus stearothermophilus ?
-
?
3.2.1.1 glycogen + H2O
-
Pyrococcus furiosus ?
-
?
3.2.1.1 glycogen + H2O
-
Bacillus licheniformis ?
-
?
3.2.1.1 glycogen + H2O
-
Bacillus amyloliquefaciens ?
-
?
3.2.1.1 glycogen + H2O
-
Rhodothermus marinus ?
-
?
3.2.1.1 glycogen + H2O
-
Pyrococcus woesei ?
-
?
3.2.1.1 glycogen + H2O
-
Thermotoga maritima ?
-
?
3.2.1.1 glycogen + H2O
-
Lederbergia lentus ?
-
?
3.2.1.1 glycogen + H2O
-
Thermoactinomyces vulgaris ?
-
?
3.2.1.1 glycogen + H2O
-
Rhizopus sp. ?
-
?
3.2.1.1 glycogen + H2O
-
Thermomyces lanuginosus ?
-
?
3.2.1.1 glycogen + H2O
-
Thermococcus litoralis ?
-
?
3.2.1.1 glycogen + H2O
-
Thermothelomyces heterothallicus ?
-
?
3.2.1.1 glycogen + H2O
-
Chloroflexus aurantiacus ?
-
?
3.2.1.1 glycogen + H2O
-
Thermus filiformis ?
-
?
3.2.1.1 glycogen + H2O
-
Mycothermus thermophilus ?
-
?
3.2.1.1 glycogen + H2O
-
Lipomyces kononenkoae ?
-
?
3.2.1.1 glycogen + H2O
-
Alicyclobacillus acidocaldarius ?
-
?
3.2.1.1 glycogen + H2O
-
Dictyoglomus thermophilum ?
-
?
3.2.1.1 glycogen + H2O
-
Thermococcus hydrothermalis ?
-
?
3.2.1.1 glycogen + H2O
-
Desulfurococcus mucosus ?
-
?
3.2.1.1 glycogen + H2O
-
Anoxybacillus flavithermus ?
-
?
3.2.1.1 glycogen + H2O
-
Thermococcus profundus ?
-
?
3.2.1.1 glycogen + H2O
-
Pyrodictium abyssi ?
-
?
3.2.1.1 glycogen + H2O
-
Thermococcus fumicolans ?
-
?
3.2.1.1 glycogen + H2O
-
Staphylothermus marinus ?
-
?
3.2.1.1 glycogen + H2O
-
Lactobacillus amylovorus ?
-
?
3.2.1.1 glycogen + H2O
-
Halothermothrix orenii ?
-
?
3.2.1.1 glycogen + H2O
-
Thermococcus aggregans ?
-
?
3.2.1.1 glycogen + H2O
-
Thermococcus celer ?
-
?
3.2.1.1 glycogen + H2O
-
Thermococcus guaymasensis ?
-
?
3.2.1.1 starch + H2O
-
Lactiplantibacillus plantarum malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Bacillus subtilis malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Geobacillus stearothermophilus malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Pyrococcus furiosus malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Bacillus licheniformis malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Bacillus amyloliquefaciens malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Rhodothermus marinus malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Pyrococcus woesei malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Thermotoga maritima malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Lederbergia lentus malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Thermoactinomyces vulgaris malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Rhizopus sp. malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Thermomyces lanuginosus malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Thermococcus litoralis malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Thermothelomyces heterothallicus malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Chloroflexus aurantiacus malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Thermus filiformis malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Mycothermus thermophilus malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Lipomyces kononenkoae malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Alicyclobacillus acidocaldarius malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Dictyoglomus thermophilum malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Thermococcus hydrothermalis malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Desulfurococcus mucosus malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Anoxybacillus flavithermus malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Thermococcus profundus malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Pyrodictium abyssi malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Thermococcus fumicolans malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Staphylothermus marinus malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Lactobacillus amylovorus malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Halothermothrix orenii malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Thermococcus aggregans malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Thermococcus celer malto-oligosaccharides
-
?
3.2.1.1 starch + H2O
-
Thermococcus guaymasensis malto-oligosaccharides
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 monomer 1 * 58000 Bacillus licheniformis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Lactiplantibacillus plantarum
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Bacillus subtilis
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Geobacillus stearothermophilus
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Pyrococcus furiosus
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Bacillus licheniformis
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Bacillus amyloliquefaciens
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Rhodothermus marinus
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Pyrococcus woesei
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Thermotoga maritima
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Lederbergia lentus
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Thermoactinomyces vulgaris
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Rhizopus sp.
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Thermomyces lanuginosus
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Thermococcus litoralis
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Thermothelomyces heterothallicus
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Chloroflexus aurantiacus
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Thermus filiformis
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Mycothermus thermophilus
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Lipomyces kononenkoae
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Alicyclobacillus acidocaldarius
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Dictyoglomus thermophilum
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Thermococcus hydrothermalis
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Desulfurococcus mucosus
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Anoxybacillus flavithermus
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Thermococcus profundus
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Pyrodictium abyssi
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Thermococcus fumicolans
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Staphylothermus marinus
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Lactobacillus amylovorus
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Halothermothrix orenii
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Thermococcus aggregans
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Thermococcus celer
3.2.1.1 alpha-1,4-glucan-4-glucanohydrolase
-
Thermococcus guaymasensis
3.2.1.1 Amy
-
Alicyclobacillus acidocaldarius
3.2.1.1 Amy
-
Anoxybacillus flavithermus
3.2.1.1 Amy B
-
Halothermothrix orenii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 60
-
-
Thermomyces lanuginosus
3.2.1.1 60
-
-
Mycothermus thermophilus
3.2.1.1 60
-
-
Anoxybacillus flavithermus
3.2.1.1 60
-
-
Halothermothrix orenii
3.2.1.1 60 65
-
Rhizopus sp.
3.2.1.1 60 65
-
Lactobacillus amylovorus
3.2.1.1 61
-
-
Rhodothermus marinus
3.2.1.1 62.5
-
-
Thermoactinomyces vulgaris
3.2.1.1 65
-
-
Lactiplantibacillus plantarum
3.2.1.1 65
-
-
Staphylothermus marinus
3.2.1.1 70
-
-
Bacillus subtilis
3.2.1.1 70
-
-
Bacillus amyloliquefaciens
3.2.1.1 70
-
-
Lederbergia lentus
3.2.1.1 70
-
-
Lipomyces kononenkoae
3.2.1.1 70 80
-
Geobacillus stearothermophilus
3.2.1.1 71
-
-
Chloroflexus aurantiacus
3.2.1.1 75
-
-
Alicyclobacillus acidocaldarius
3.2.1.1 80
-
-
Thermococcus profundus
3.2.1.1 85
-
-
Thermococcus hydrothermalis
3.2.1.1 85 90
-
Thermotoga maritima
3.2.1.1 88
-
-
Thermococcus litoralis
3.2.1.1 90
-
-
Dictyoglomus thermophilum
3.2.1.1 90
-
-
Thermococcus celer
3.2.1.1 95
-
-
Thermus filiformis
3.2.1.1 95
-
-
Thermococcus fumicolans
3.2.1.1 100
-
-
Pyrococcus furiosus
3.2.1.1 100
-
-
Bacillus licheniformis
3.2.1.1 100
-
-
Pyrococcus woesei
3.2.1.1 100
-
-
Thermothelomyces heterothallicus
3.2.1.1 100
-
-
Desulfurococcus mucosus
3.2.1.1 100
-
-
Pyrodictium abyssi
3.2.1.1 100
-
-
Thermococcus aggregans
3.2.1.1 100
-
-
Thermococcus guaymasensis