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Literature summary extracted from

  • Kouakou, T.H.; Kouadio, Y.J.; Kouame, P.; Waffo-Teguo, P.; Decendit, A.; Merillon, J.M.
    Purification and biochemical characterization of polyphenol oxidases from embryogenic and nonembryogenic cotton (Gossypium hirsutum L.) cells (2009), Appl. Biochem. Biotechnol., 158, 285-301.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.1 additional information highest activity is measured using the following buffer composition: sodium phosphate + Triton X-100 (1%) + PVP (0.5%) + EDTA (1.0 mM) + sodium thiosulfate (0.25%) Gossypium hirsutum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 ascorbic acid markedly inhibits PPO Gossypium hirsutum
1.10.3.1 Ba2+ moderately inhibits PPO Gossypium hirsutum
1.10.3.1 Cu2+ moderately inhibits PPO Gossypium hirsutum
1.10.3.1 dithiothreitol markedly inhibits PPO Gossypium hirsutum
1.10.3.1 FeCl3 markedly inhibits PPO Gossypium hirsutum
1.10.3.1 SnCl2 markedly inhibits PPO Gossypium hirsutum
1.10.3.1 Sodium metabisulfite markedly inhibits PPO Gossypium hirsutum
1.10.3.1 Zn2+ moderately inhibits PPO Gossypium hirsutum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.1 Ca2+ highly activates PPO Gossypium hirsutum
1.10.3.1 Mg2+ highly activates PPO Gossypium hirsutum
1.10.3.1 Mn2+ highly activates PPO Gossypium hirsutum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 55000
-
SDS-PAGE Gossypium hirsutum

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Gossypium hirsutum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 using a Sephadex G-200 column balanced with 0.1 M sodium phosphate buffer (pH 6.5) Gossypium hirsutum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 cell suspension culture coker 312 seeds are used. Cotton cell suspensions are obtained from calli derived from hypocotyl segments of plant. Coker 312 cell suspensions show the development of embryogenic structures Gossypium hirsutum
-
1.10.3.1 cell suspension culture R405-2000 seeds are used. Cotton cell suspensions are obtained from calli derived from hypocotyl segments of plant. R405-2000 cell suspensions does not show the development of embryogenic structures Gossypium hirsutum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 additional information
-
compared to PPO from R405-2000, a nonembryogenic cultivar, PPO from coker 312 (embryogenic cultivar) shows a higher PPO activity increasing markedly form primary culture to third subculture Gossypium hirsutum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 2 catechol + O2 enzyme shows high affinity to this substrate Gossypium hirsutum 2 1,2-benzoquinone + 2 H2O
-
?
1.10.3.1 2 catechol + O2 enzyme shows highest affinity to this substrate Gossypium hirsutum 2 1,2-benzoquinone + 2 H2O
-
?
1.10.3.1 4-methylcatechol + O2 enzyme shows high affinity to this substrate Gossypium hirsutum ?
-
?
1.10.3.1 4-methylcatechol + O2 enzyme shows highest affinity to this substrate Gossypium hirsutum ?
-
?
1.10.3.1 catechin + O2 enzyme shows moderate affinity to this substrate Gossypium hirsutum ?
-
?
1.10.3.1 dopamine + O2 enzyme shows moderate affinity to this substrate Gossypium hirsutum ?
-
?
1.10.3.1 gallic acid + O2 enzyme shows very low affinity to this substrate Gossypium hirsutum ?
-
?
1.10.3.1 additional information PPO shows activity with biphenols, triphenol but not with monophenols Gossypium hirsutum ?
-
?
1.10.3.1 pyrogallol + O2 enzyme shows low affinity to this substrate Gossypium hirsutum ?
-
?
1.10.3.1 tetramethylbenzidine + O2 enzyme shows low affinity to this substrate Gossypium hirsutum ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 polyphenol oxidase
-
Gossypium hirsutum
1.10.3.1 PPO from Coker 312, an embryogenic cultivar Gossypium hirsutum
1.10.3.1 PPO from R405-2000, an nonembryogenic cultivar Gossypium hirsutum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 25
-
-
Gossypium hirsutum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.1 20 30 60 min Gossypium hirsutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 6
-
optimal pH, highest activity is measured using the following buffer composition: sodium phosphate + Triton X-100 (1%) + PVP (0.5%) + EDTA (1.0 mM) + sodium thiosulfate (0.25%) Gossypium hirsutum
1.10.3.1 6.5
-
assay at Gossypium hirsutum
1.10.3.1 7
-
optimal pH, highest activity is measured using the following buffer composition: sodium phosphate + Triton X-100 (1%) + PVP (0.5%) + EDTA (1.0 mM) + sodium thiosulfate (0.25%) Gossypium hirsutum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.1 5 6 enzyme retains 95% of its activity Gossypium hirsutum
1.10.3.1 6 7 enzyme retains 95% of its activity Gossypium hirsutum