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Literature summary extracted from

  • Zeng, Y.; Roy, H.; Patil, P.B.; Ibba, M.; Chen, S.
    Characterization of two seryl-tRNA synthetases in albomycin-producing Streptomyces sp. strain ATCC 700974 (2009), Antimicrob. Agents Chemother., 53, 4619-4627.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.11 into the vectors pET30a+ and pUC19 Streptomyces sp.
6.1.1.11 into the vectors pET30a+, pSUP and pUC19, the H270G PCR product is cloned into the vector pET15b Streptomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.11 H270G SerRS2 mutant Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.11 SB-217452
-
 Streptomyces sp.
6.1.1.11 serine hydroxamate
-
 Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.11 0.0086
-
 tRNASer 37°C, aminoacylation Streptomyces sp.
6.1.1.11 0.012
-
 tRNASer 30°C, aminoacylation Streptomyces sp.
6.1.1.11 0.24
-
 tRNASer aminoacylation Streptomyces sp.
6.1.1.11 0.26
-
 L-serine diphosphate exchange Streptomyces sp.
6.1.1.11 0.4
-
 L-serine diphosphate exchange Streptomyces sp.
6.1.1.11 0.41
-
 tRNASer H270G mutant, aminoacylation Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer Streptomyces sp.
-
 AMP + diphosphate + L-seryl-tRNASer
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.11 Streptomyces sp. D0UD38 strain ATCC 700974
-
6.1.1.11 Streptomyces sp. D0UD39 strain ATCC 700974
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.11 carried out with Ni-nitriloacetic acid spin columns Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer
-
 Streptomyces sp. AMP + diphosphate + L-seryl-tRNASer
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.11 SerRS1
-
 Streptomyces sp.
6.1.1.11 SerRS2
-
 Streptomyces sp.
6.1.1.11 seryl-tRNA synthetase 1
-
 Streptomyces sp.
6.1.1.11 seryl-tRNA synthetase 2
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.11 30
-
 aminoacylation assay Streptomyces sp.
6.1.1.11 37
-
 aminoacylation assay Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.11 0.057
-
 tRNASer 30°C, aminoacylation Streptomyces sp.
6.1.1.11 0.125
-
 tRNASer 37°C, aminoacylation Streptomyces sp.
6.1.1.11 0.18
-
 tRNASer H270G mutant, aminoacylation Streptomyces sp.
6.1.1.11 1.37
-
 tRNASer aminoacylation Streptomyces sp.
6.1.1.11 1.8
-
 L-serine diphosphate exchange Streptomyces sp.
6.1.1.11 7
-
 L-serine diphosphate exchange Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.11 7.2
-
 aminoacylation assay Streptomyces sp.

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.11 ATP
-
 Streptomyces sp.