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Literature summary extracted from

  • Caballero, I.; Piedrahita, J.A.
    Evaluation of the Serratia marcescens nuclease (NucA) as a transgenic cell ablation system in porcine (2009), Anim. Biotechnol., 20, 177-185.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.30.2 construct containing the NucA gene driven by the beta-actin promoter coupled with enhancer elements from the cytomegalovirus promoter and rabbit beta-globin gene and the blasticidin resistance gene driven by the phosphoglycerate kinase promoter are generated and electroporated into porcine fetal fibroblasts Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.2 Serratia marcescens P13717
-
-

Synonyms

EC Number Synonyms Comment Organism
3.1.30.2 nucA
-
 Serratia marcescens
3.1.30.2 NucANLS
-
 Serratia marcescens
3.1.30.2 NucANLS gene with a nuclear localization signal Serratia marcescens
3.1.30.2 Serratia marcescens nuclease
-
 Serratia marcescens

Expression

EC Number Organism Comment Expression
3.1.30.2 Serratia marcescens both NucA with and without a nuclear localization signal prove to be effective in killing porcine fibroblasts compared to controls, gene expression analysis of surviving colonies indicates that survival is related to low or absent expression of the toxic genes additional information

General Information

EC Number General Information Comment Organism
3.1.30.2 physiological function enzyme is able to ablate cells in culture Serratia marcescens