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Literature summary extracted from
- Crystallization of Mycobacterium smegmatis methionyl-tRNA synthetase in the presence of methionine and adenosine (2009), Acta Crystallogr. Sect. F, 65, 618-620.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 6.1.1.10 | into the vector pET101/D-TOPO, a His-Tag is attached, for expression in Escherichia coli BL21 StarDE3 cells | Mycolicibacterium smegmatis |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 6.1.1.10 | the structure of methionyl-tRNA synthetase is determined to a resolution of 2.1 A | Mycolicibacterium smegmatis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.10 | 58000 | - |
determined by SDS-PAGE | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.1.1.10 | ATP + L-methionine + tRNAMet | Mycolicibacterium smegmatis | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.1.1.10 | Mycolicibacterium smegmatis | A0R3E2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.1.1.10 | Ni-NTA agarose is used, in addition gel filtration on a HiLoad 16/60 Superdex-200 column is applied | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.1.1.10 | ATP + L-methionine + tRNAMet | - |
Mycolicibacterium smegmatis | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 6.1.1.10 | monomer | - |
Mycolicibacterium smegmatis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 6.1.1.10 | methionyl-tRNA synthetase | - |
Mycolicibacterium smegmatis |
| 6.1.1.10 | MetRS | - |
Mycolicibacterium smegmatis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.1.1.10 | ATP | - |
Mycolicibacterium smegmatis | |
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Release 2023.1 (January 2023)
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