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Literature summary extracted from

  • Padmanabhan, B.; Strange, R.W.; Antonyuk, S.V.; Ellis, M.J.; Hasnain, S.S.; Iino, H.; Agari, Y.; Bessho, Y.; Yokoyama, S.
    Structure of dihydrodipicolinate synthase from Methanocaldococcus jannaschii (2009), Acta Crystallogr. Sect. F, 65, 1222-1226.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.7 into the pET-21a expression vector and introduced into Escherichia coli Rosetta (DE3) strain Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.3.7 by the oil-batch method at 291 K, to 2.2 A resolution. Belongs to space group P21 with unit-cell parameters a = 80.5 A, b = 76.5 A, c = 101.9 A, gamma = 106.9 A. The asymmetric unit contains four DHDPS molecules, forming a homotetramer with approximate 222 symmetry. The overall tertiary structure of DHDPS possesses a (beta/alpha)8-barrel fold (TIM barrel) with three additional alpha-helices (alpha9-alpha11) at the C-terminus of the chain. The beta-strands of the barrel form an intrinsic network of hydrogen-bonding interactions with the neighbouring beta-strands and are oriented in the same directions. The functional residue Lys161, which participates in Schiff-base formation, is located within the beta-barrel and the side chain of Tyr132 sits over this residue Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.7 Methanocaldococcus jannaschii Q57695
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Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.7 by gel filtration and ultrafiltration Methanocaldococcus jannaschii

Subunits

EC Number Subunits Comment Organism
4.3.3.7 homotetramer crystallography Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
4.3.3.7 DHDPS
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 Methanocaldococcus jannaschii
4.3.3.7 dihydrodipicolinate synthase
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 Methanocaldococcus jannaschii