EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.45 | expressed in Escherichia coli BL21(DE3) cells | Trypanosoma cruzi |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.1.45 | in complexes with the dihydrotriazine-based or quinazoline-based antifolates C-448, cycloguanil, and Q-8, microbatch method, using 0.1-0.2 M ammonium acetate, 20-22% (w/v) PEG 4000 and 0.1 M sodium citrate buffer pH 5.6 | Trypanosoma cruzi |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 58800 | - |
calculated from amino acid sequence | Trypanosoma cruzi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.45 | Trypanosoma cruzi | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.45 | methotrexate affinity column chromatography | Trypanosoma cruzi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma cruzi | dihydrofolate + dTMP | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.45 | DHFR-TS | - |
Trypanosoma cruzi |
2.1.1.45 | dihydrofolate reductase-thymidylate synthase | - |
Trypanosoma cruzi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | N5,N10-methylenetetrahydrofolate | - |
Trypanosoma cruzi |