Literature summary extracted from
Cicmil, N.
Crystallization and preliminary X-ray crystallographic characterization of TrmFO, a folate-dependent tRNA methyltransferase from Thermotoga maritima (2008), Acta Crystallogr. Sect. F, 64, 193-195.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.1.1.74 |
overexpression in Escherichia coli strain BL21(DE3) |
Thermotoga maritima |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.1.1.74 |
purified recombinant enzyme, from 25% v/v PEG 4000, 100 mM NaCl and sodium citrate buffer, pH 5.0, at 291 K using the hanging-drop vapor-diffusion method in 0.0016 ml with 8 mg/ml protein, 7 days, X-ray diffraction structure determination and analysis at 2.6 A resolution |
Thermotoga maritima |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.1.1.74 |
5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 |
Thermotoga maritima |
TrmFO is a methyltransferase that catalyzes the formation of 5-methyluridine or ribothymidine at position 54 in tRNA in some Gram-positive bacteria |
tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.1.1.74 |
Thermotoga maritima |
- |
gene trmFO |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.1.1.74 |
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion and cation exchange chromatography and gel filtration to over 95% purity |
Thermotoga maritima |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.1.1.74 |
5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 |
TrmFO is a methyltransferase that catalyzes the formation of 5-methyluridine or ribothymidine at position 54 in tRNA in some Gram-positive bacteria |
Thermotoga maritima |
tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD |
- |
? |
|
2.1.1.74 |
N5,N10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 |
- |
Thermotoga maritima |
tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.1.1.74 |
TRMFO |
- |
Thermotoga maritima |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.1.1.74 |
FAD |
dependent on |
Thermotoga maritima |
|