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Literature summary extracted from
- Structures of mannose-6-phosphate isomerase from Salmonella typhimurium bound to metal atoms and substrate: implications for catalytic mechanism (2009), Acta Crystallogr. Sect. D, 65, 724-732.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.8 | overexpression of the N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.3.1.8 | enzyme in apoform without metal ion, in the holoform with bound Zn2+, or complexed with bound inhibitor yttrium, or with Zn2+ and fructose 6-phosphate, microbatch method, 0.003 ml of protein solution and crystallization solution are mixed containing 4 mg/ml protein, 0.1 M magnesium acetate, 0.2 M sodium cacodylate, pH 6.5, 20% PEG 8000 and 5% dioxane, addition of 10 mM metal ions and 250 mM fructose 6-phosphate for complexed enzyme, X-ray diffraction structure determination and analysis at 1.7-2.5 A resolution, molecular replacement | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.8 | EDTA | complete inhibition at 1 mM, reversible by the addition of divalent metal cations such as Zn2+, Mn2+, Co2+, Mg2+ and Ni2+ | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 5.3.1.8 | Y3+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.8 | Ca2+ | activates | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 5.3.1.8 | Co2+ | activates | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 5.3.1.8 | Mg2+ | best activating divalent metal ion | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 5.3.1.8 | Mn2+ | activates | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 5.3.1.8 | additional information | divalent cations are absolutely required for activity, metal binding increases the thermostability of the enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 5.3.1.8 | Ni2+ | activates | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 5.3.1.8 | Zn2+ | enzyme-bound, Zn2+ binding induces structural order in the loop consisting of residues 50-54. The metal atom appears to play a role in substrate binding and is probably also important for maintaining the architecture of the active site | Salmonella enterica subsp. enterica serovar Typhimurium | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.8 | D-Mannose 6-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
D-Fructose 6-phosphate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.8 | Salmonella enterica subsp. enterica serovar Typhimurium | P25081 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.8 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 5.3.1.8 | D-Mannose 6-phosphate = D-fructose 6-phosphate | reaction mechanism, overview. Zn2+ binding induces structural order in the loop consisting of residues 50-54. The metal atom appears to play a role in substrate binding and is probably also important for maintaining the architecture of the active site. Isomerization probably follows a cis-enediol mechanism, overall folding pattern of the central catalytic domain, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.8 | D-Mannose 6-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | D-Fructose 6-phosphate | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.1.8 | MPI | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.8 | 22 | - |
assay at room temperature | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.8 | 8.5 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.8 | 6.5 | 9 | activity range | Salmonella enterica subsp. enterica serovar Typhimurium |
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Release 2023.1 (January 2023)
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