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Literature summary extracted from

  • Chungool, W.; Thongkam, W.; Raweesri, P.; Thamchaipenet, A.; Pinphanichakarn, P.
    Production, purification, and characterization of acetyl esterase from Streptomyces sp. PC22 and its action in cooperation with xylanolytic enzymes on xylan degradation (2008), World J. Microbiol. Biotechnol., 24, 549-556.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.6 industry cooperative action on birchwood xylan degradation in combination with Streptomyces sp. xylanase and beta xylosidase, production of biofuels utilizing hemicelluloses containing acetyl xylan, 1.16fold to 1.39fold increased sugar release Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.6 Cu2+ acetyl esterase activity Streptomyces sp.
3.1.1.6 Hg2+ strong inhibitor, 0.1 mM, acetyl esterase activity Streptomyces sp.
3.1.1.6 additional information 10 mM ethylmethylaminopropyl carbodiimide or iodacetamide without effect on acetyl esterase activity Streptomyces sp.
3.1.1.6 phenyl methylsulfonyl fluoride 10 mM, 50% decreased acetyl esterase activity Streptomyces sp.
3.1.1.6 Zn2+ acetyl esterase activity Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.6 0.43
-
 p-nitrophenyl acetate pH 6.5 Streptomyces sp.
3.1.1.6 7.8
-
 Alpha-naphthyl acetate pH 6.5 Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.6 additional information 10 mM Ca2+, Co2+, Mg2+ or Mn2+ without any affect on acetyl esterase activity Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.6 38000
-
 SDS-PAGE Streptomyces sp.
3.1.1.6 155000
-
 native, gel-filtration on Sephacryl S-200 HR column Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.6 Streptomyces sp.
-
 PC22
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.6 at 4°C, precipitation from culture filtrate by 40-80% (NH4)2SO4 saturation followed by anion-exchange (DEAE column, elution with 0-1 M NaCl gradient), t-butyl hydrophobic (elution with 1.7-0 M (NH4)2SO4 gradient) and hydroxyapatite chromatography (elution with 0.02-0.5 M sodium phosphate buffer, pH 6.8), stored at -20°C after addition of 20% glycerol Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.6 9.63
-
 p-nitrophenyl acetate, after hydroxyapatite chromatography, pH 6.5 Streptomyces sp.
3.1.1.6 71
-
 p-nitrophenyl acetate, pH 6.5 Streptomyces sp.
3.1.1.6 1027
-
 alpha-naphthyl acetate, pH 6.5 Streptomyces sp.

Storage Stability

EC Number Storage Stability Organism
3.1.1.6 stored at -20°C in 20% glycerol Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.6 acetylxylan + H2O 60°C, pH 6, acetyl xylan esterase (acetate-releasing) activity on birchwood xylan Streptomyces sp. xylan + acetate
-
 ?
3.1.1.6 alpha-naphthyl acetate + H2O 15 min, 50°C, pH 6.5 Streptomyces sp. alpha-naphthol + acetate
-
 ?
3.1.1.6 additional information no activity on p-nitrophenyl-beta-D-xylopyranoside and p-nitrophenyl-alpha-L-arabinofuranoside and no xylanolytic activity Streptomyces sp. ?
-
 ?
3.1.1.6 p-nitrophenyl acetate + H2O 10 min, 50°C, pH 6.5 Streptomyces sp. p-nitrophenol + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.6 tetramer 4 * 38000, gel filtration on Sephacryl S-200 HR column Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.1.6 Acetyl esterase
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.6 50
-
-
 Streptomyces sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.6 40 65
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.6 60
-
 stable for 30 min at temperatures up to 60°C in absence of substrate Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.6 6
-
 85% residual activity, alpha-naphthyl acetate as substrate, more active in acetate buffer than phosphate buffer Streptomyces sp.
3.1.1.6 6.5 7 optimum activity, alpha-naphthyl acetate as substrate Streptomyces sp.
3.1.1.6 8
-
 65% residual activity, alpha-naphthyl acetate as substrate Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.6 5 9 stable for 30 min Streptomyces sp.