EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.1.6 | industry | cooperative action on birchwood xylan degradation in combination with Streptomyces sp. xylanase and beta xylosidase, production of biofuels utilizing hemicelluloses containing acetyl xylan, 1.16fold to 1.39fold increased sugar release | Streptomyces sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.6 | Cu2+ | acetyl esterase activity | Streptomyces sp. | |
3.1.1.6 | Hg2+ | strong inhibitor, 0.1 mM, acetyl esterase activity | Streptomyces sp. | |
3.1.1.6 | additional information | 10 mM ethylmethylaminopropyl carbodiimide or iodacetamide without effect on acetyl esterase activity | Streptomyces sp. | |
3.1.1.6 | phenyl methylsulfonyl fluoride | 10 mM, 50% decreased acetyl esterase activity | Streptomyces sp. | |
3.1.1.6 | Zn2+ | acetyl esterase activity | Streptomyces sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.6 | 0.43 | - |
p-nitrophenyl acetate | pH 6.5 | Streptomyces sp. | |
3.1.1.6 | 7.8 | - |
Alpha-naphthyl acetate | pH 6.5 | Streptomyces sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.6 | additional information | 10 mM Ca2+, Co2+, Mg2+ or Mn2+ without any affect on acetyl esterase activity | Streptomyces sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.6 | 38000 | - |
SDS-PAGE | Streptomyces sp. |
3.1.1.6 | 155000 | - |
native, gel-filtration on Sephacryl S-200 HR column | Streptomyces sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.6 | Streptomyces sp. | - |
PC22 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.6 | at 4°C, precipitation from culture filtrate by 40-80% (NH4)2SO4 saturation followed by anion-exchange (DEAE column, elution with 0-1 M NaCl gradient), t-butyl hydrophobic (elution with 1.7-0 M (NH4)2SO4 gradient) and hydroxyapatite chromatography (elution with 0.02-0.5 M sodium phosphate buffer, pH 6.8), stored at -20°C after addition of 20% glycerol | Streptomyces sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.6 | 9.63 | - |
p-nitrophenyl acetate, after hydroxyapatite chromatography, pH 6.5 | Streptomyces sp. |
3.1.1.6 | 71 | - |
p-nitrophenyl acetate, pH 6.5 | Streptomyces sp. |
3.1.1.6 | 1027 | - |
alpha-naphthyl acetate, pH 6.5 | Streptomyces sp. |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.1.6 | stored at -20°C in 20% glycerol | Streptomyces sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.6 | acetylxylan + H2O | 60°C, pH 6, acetyl xylan esterase (acetate-releasing) activity on birchwood xylan | Streptomyces sp. | xylan + acetate | - |
? | |
3.1.1.6 | alpha-naphthyl acetate + H2O | 15 min, 50°C, pH 6.5 | Streptomyces sp. | alpha-naphthol + acetate | - |
? | |
3.1.1.6 | additional information | no activity on p-nitrophenyl-beta-D-xylopyranoside and p-nitrophenyl-alpha-L-arabinofuranoside and no xylanolytic activity | Streptomyces sp. | ? | - |
? | |
3.1.1.6 | p-nitrophenyl acetate + H2O | 10 min, 50°C, pH 6.5 | Streptomyces sp. | p-nitrophenol + acetate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.6 | tetramer | 4 * 38000, gel filtration on Sephacryl S-200 HR column | Streptomyces sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.6 | Acetyl esterase | - |
Streptomyces sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.6 | 50 | - |
- |
Streptomyces sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.6 | 40 | 65 | - |
Streptomyces sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.6 | 60 | - |
stable for 30 min at temperatures up to 60°C in absence of substrate | Streptomyces sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.6 | 6 | - |
85% residual activity, alpha-naphthyl acetate as substrate, more active in acetate buffer than phosphate buffer | Streptomyces sp. |
3.1.1.6 | 6.5 | 7 | optimum activity, alpha-naphthyl acetate as substrate | Streptomyces sp. |
3.1.1.6 | 8 | - |
65% residual activity, alpha-naphthyl acetate as substrate | Streptomyces sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.6 | 5 | 9 | stable for 30 min | Streptomyces sp. |