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Literature summary extracted from

  • AbuBakr, S.; Macmil, S.; Nanny, M.; Duncan, K.
    Enzymatic transformation of humic substances by NDO (2008), Soil Biol. Biochem., 40, 2055-2062.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.12 His-tagged, expressed in Escherichia coli Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.12 Pseudomonas putida
-
G7
-
1.14.12.12 Pseudomonas putida G7
-
G7
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.12 metal affinity chromatography Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.14.12.12 23-25°C, buffer, 14 days, loss of about 80% activity Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.12 fulvic acid + NADH + H+ + O2
-
Pseudomonas putida ?
-
?
1.14.12.12 fulvic acid + NADH + H+ + O2
-
Pseudomonas putida G7 ?
-
?
1.14.12.12 humic acid + NADH + H+ + O2
-
Pseudomonas putida ?
-
?
1.14.12.12 humic acid + NADH + H+ + O2
-
Pseudomonas putida G7 ?
-
?
1.14.12.12 indole + NADH + H+ + O2
-
Pseudomonas putida ?
-
?
1.14.12.12 indole + NADH + H+ + O2
-
Pseudomonas putida G7 ?
-
?
1.14.12.12 naphthalene + NADH + H+ + O2
-
Pseudomonas putida 1,2-dihydronaphthalene-1,2-diol + NAD+
-
?
1.14.12.12 naphthalene + NADH + H+ + O2
-
Pseudomonas putida G7 1,2-dihydronaphthalene-1,2-diol + NAD+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.12 naphthalene 1,2-dioxygenase
-
Pseudomonas putida