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Literature summary extracted from

  • Gou, M.; Qu, Y.; Zhou, J.; Li, A.; Salah Uddin, M.
    Characterization of catechol 1,2-dioxygenase from cell extracts of Sphingomonas xenophaga QYY (2009), Sci. China, Ser. B, Chem. Life Sci. Earth Sci., 52, 615-620.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.1 additional information inducible enzyme, strain QYY synthesize C12O only when it grow on culture in the presence of catechol Sphingobium xenophagum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.05285
-
 catechol
-
 Sphingobium xenophagum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Ba2+ 1 mmol/l, 30°C, 15 min, 106% remaining activity Sphingobium xenophagum
1.13.11.1 Ca2+ 1 mmol/l, 30°C, 15 min, 168% remaining activity Sphingobium xenophagum
1.13.11.1 Co2+ 1 mmol/l, 30°C, 15 min, 52.7% remaining activity Sphingobium xenophagum
1.13.11.1 Cu2+ 1 mmol/l, 30°C, 15 min, completely inactivated Sphingobium xenophagum
1.13.11.1 Fe2+ 1 mmol/l, 30°C, 15 min, completely inactivated Sphingobium xenophagum
1.13.11.1 Fe3+ 1 mmol/l, 30°C, 15 min, ferric-dependent enzyme, 140% remaining activity Sphingobium xenophagum
1.13.11.1 Hg2+ 1 mmol/l, 30°C, 15 min, 76.2% remaining activity Sphingobium xenophagum
1.13.11.1 K+ 1 mmol/l, 30°C, 15 min, 1317% remaining activity Sphingobium xenophagum
1.13.11.1 Mg2+ 1 mmol/l, 30°C, 15 min, 190.7% remaining activity Sphingobium xenophagum
1.13.11.1 Mn2+ 1 mmol/l, 30°C, 15 min, 89.7% remaining activity Sphingobium xenophagum
1.13.11.1 Ni2+ 1 mmol/l, 30°C, 15 min, 74.6% remaining activity Sphingobium xenophagum
1.13.11.1 Pb2+ 1 mmol/l, 30°C, 15 min, 281% remaining activity Sphingobium xenophagum
1.13.11.1 Zn2+ 1 mmol/l, 30°C, 15 min, 2% remaining activity Sphingobium xenophagum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 35000
-
 SDS-PAGE, value about Sphingobium xenophagum

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Sphingobium xenophagum
-
-
-
1.13.11.1 Sphingobium xenophagum QYY
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1 partial purification with HiTrap Q Sepharose column chromatography Sphingobium xenophagum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 0.02
-
 growth condition: LB medium added with 500mg/l catechol Sphingobium xenophagum
1.13.11.1 0.5
-
 growth condition: catechol Sphingobium xenophagum
1.13.11.1 1.14
-
 growth condition: tryptone medium added with 500 mg/l catechol Sphingobium xenophagum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 catechol + ?
-
 Sphingobium xenophagum ?
-
 ?
1.13.11.1 catechol + ?
-
 Sphingobium xenophagum QYY ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.1 C12O
-
 Sphingobium xenophagum
1.13.11.1 catechol 1,2-dioxygenase
-
 Sphingobium xenophagum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.1 40 70
-
 Sphingobium xenophagum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.1 7.8 8.8 increase of enzyme activity at pH 5.8-8.8, 29.3% decrease of enzyme activity at pH 9.4, 50% decrease of enzyme activity at pH 10.7 Sphingobium xenophagum