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Literature summary extracted from

  • Eprintsev, A.; Semenov, A.; Navid, M.; Popov, V.
    Physical, chemical, and regulatory properties of glycolate oxidase in C3 and C4 plants (2009), Russ. J. Plant Physiol., 56, 164-167.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.15 additional information
-
 additional information Michaelis-Menten kinetics Zea mays
1.1.3.15 additional information
-
 additional information Michaelis-Menten kinetics Glycine max
1.1.3.15 0.02
-
 glycolate mesophyll cell enzyme Zea mays
1.1.3.15 0.056
-
 glycolate bundle sheath cell enzyme Zea mays
1.1.3.15 0.06
-
 glycolate leaf enzyme Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.15 44000
-
 2 x 37000 + 2 * 44000, SDS-PAGE Zea mays
1.1.3.15 160000
-
 sequence calculation Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.15 glycolate + O2 Zea mays
-
 glyoxylate + H2O2
-
 ?
1.1.3.15 glycolate + O2 Glycine max
-
 glyoxylate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Glycine max
-
 cv. Pripyat, various isozymes
-
1.1.3.15 Zea mays
-
 cv. Voronezhskaya 76, various isozymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.15 native enzyme from leaves 207fold by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and again gel filtration Glycine max
1.1.3.15 native enzyme from maize leaf bundle sheath 46.6fold by gel filtration, anion exchange chromatography, and again gel filtration. Native enzyme 10.5fold from leaf mesophyll, by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and again gel filtration Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.15 bundle sheath
-
 Zea mays
-
1.1.3.15 leaf
-
 Zea mays
-
1.1.3.15 leaf
-
 Glycine max
-
1.1.3.15 mesophyll
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.15 0.65
-
 purified native enzyme from leaves Zea mays
1.1.3.15 1546
-
 purified native enzyme from mesophyll bundle sheath Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 glycolate + O2
-
 Zea mays glyoxylate + H2O2
-
 ?
1.1.3.15 glycolate + O2
-
 Glycine max glyoxylate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.15 More quaternary structure of C3 plant GO, overview Zea mays
1.1.3.15 octamer
-
 Glycine max
1.1.3.15 tetramer 2 x 37000 + 2 * 44000, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
1.1.3.15 glycolate oxidase
-
 Zea mays
1.1.3.15 glycolate oxidase
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.15 additional information
-
 the dependence of GO activity on pH is bell-shaped Zea mays

General Information

EC Number General Information Comment Organism
1.1.3.15 metabolism the enzyme is involved in the metabolism of glycolate Glycine max
1.1.3.15 metabolism the enzyme is involved in the metabolism of glycolate, glycolate oxidase is a key enzyme involved in C3 photorespiration metabolic pathway, the process where plants lose up to half of assimilated carbon Zea mays
1.1.3.15 physiological function role in C3 and C4 plants and associated regulation mechanisms Zea mays
1.1.3.15 physiological function role in C3 and C4 plants and associated regulation mechanisms Glycine max