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Literature summary extracted from

  • Jin, F.L.; Xu, X.X.; Yu, X.Q.; Ren, S.X.
    High-level expression of active recombinant ubiquitin carboxyl-terminal hydrolase of Drosophila melanogaster in Pichia pastoris (2009), Protein Expr. Purif., 65, 115-121.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.19.12 molecular biology Pichia pastoris is a robust system to express the secreted form of Drosophila melanogaster UCH Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.19.12 active C-terminal His-tagged UCH expressed in Pichia pastoris Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.19.12 29000
-
 1 * 29000, SDS-PAGE or calculated from the deduced amino acid sequence Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.12 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.12 recombinant UCH purified to homogeneity by gel filtration, fusion protein purified by Ni-NTA chromatography Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.12 ubiquitin-magainin + H2O is efficiently cleaved at the carboxyl terminus of ubquitin, yielding recombinant magainin with high antimicrobial activity Drosophila melanogaster magainin + ubiquitin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.19.12 monomer 1 * 29000, SDS-PAGE or calculated from the deduced amino acid sequence Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
3.4.19.12 ubiquitin carboxyl-terminal hydrolase
-
 Drosophila melanogaster
3.4.19.12 UCH
-
 Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.12 6
-
-
 Drosophila melanogaster