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Literature summary extracted from

  • Volonte, F.; Marinelli, F.; Gastaldo, L.; Sacchi, S.; Pilone, M.S.; Pollegioni, L.; Molla, G.
    Optimization of glutaryl-7-aminocephalosporanic acid acylase expression in E. coli (2008), Protein Expr. Purif., 61, 131-137.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.93 industry the huge production of engineered GLA produced in Escherichia coli under optimized conditions makes this enzyme an economic tool to be used in bioconversion processes at the industrial level, e.g., in 7-ACA production Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.93 into the vector pET24 for expression in Escherichia coli BL21DE3 pLysS cells Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.93 26000
-
 alpha-subunit, determined by SDS-PAGE Pseudomonas sp.
3.5.1.93 58000
-
 beta-subunit, determined by SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.93 (7R)-7-(4-carboxybutanamido)cephalosporanate + H2O Pseudomonas sp.
-
 (7R)-7-aminocephalosporanate + glutarate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.93 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.93 by HiTrap chelating affinity chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.93 0.28
-
 LB Miller broth medium Pseudomonas sp.
3.5.1.93 3.7
-
 purification step crude extract Pseudomonas sp.
3.5.1.93 11.4
-
 optimized conditions, SB3 medium, 12.5 g NaCl/l, 0.6 mM IPTG Pseudomonas sp.
3.5.1.93 125
-
 purification step HiTrap chelating chromatography Pseudomonas sp.
3.5.1.93 133
-
 purification step PD10 desalting Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.93 (7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
-
 Pseudomonas sp. (7R)-7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O activity assay Pseudomonas sp. 7-aminocephalosporanic acid + glutarate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.93 heterodimer
-
 Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.1.93 glutaryl-7-aminocephalosporanic acid acylase
-
 Pseudomonas sp.