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Literature summary extracted from

  • Sadhasivam, S.; Savitha, S.; Swaminathan, K.; Lin, F.
    Production, purification and characterization of mid-redox potential laccase from a newly isolated Trichoderma harzianum WL1 (2008), Process Biochem., 43, 736-742.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 azide complete inhibition at 0.02 mM Trichoderma harzianum
1.10.3.2 Cr3+ inhibits 13.8% at 1 mM Trichoderma harzianum
1.10.3.2 diethyldithiocarbamic acid
-
 Trichoderma harzianum
1.10.3.2 EDTA
-
 Trichoderma harzianum
1.10.3.2 ethanol
-
 Trichoderma harzianum
1.10.3.2 Hg2+ inhibits 17.2% at 1 mM Trichoderma harzianum
1.10.3.2 additional information no or poor inhibition by Co2+, Zn2+, Sn2+, Mn2+, Mg2+, K+, Fe2+, Ba2+, and Na+ Trichoderma harzianum
1.10.3.2 thioglycolic acid inhibits 92.4% at 750 mM Trichoderma harzianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.06
-
 guaiacol pH 4.5, 35°C Trichoderma harzianum
1.10.3.2 0.18
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.5, 35°C Trichoderma harzianum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+
-
 Trichoderma harzianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 79000
-
 x * 79000, SDS-PAGE Trichoderma harzianum

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Trichoderma harzianum
-
-
-
1.10.3.2 Trichoderma harzianum WL1
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein
-
 Trichoderma harzianum

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme 151.7fold by ultrafiltration, gel filtration, and concanavalin A affinity chromatography Trichoderma harzianum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 130.5
-
 purified enzyme Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trichoderma harzianum ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trichoderma harzianum WL1 ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Trichoderma harzianum ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Trichoderma harzianum WL1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 79000, SDS-PAGE Trichoderma harzianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 35
-
-
 Trichoderma harzianum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 30 50 activity range, rapid loss at 60°C Trichoderma harzianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 35
-
 purified enzyme, 24 h, stable Trichoderma harzianum
1.10.3.2 65
-
 purified enzyme, half-life is 60 min Trichoderma harzianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4.5
-
-
 Trichoderma harzianum