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Literature summary extracted from

  • Oberhauser, V.; Voolstra, O.; Bangert, A.; von Lintig, J.; Vogt, K.
    NinaB combines carotenoid oxygenase and retinoid isomerase activity in a single polypeptide (2008), Proc. Natl. Acad. Sci. USA, 105, 19000-19005.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.63 for expression in Escherichia coli cells, genetically engineered to produce beta-carotene Mus musculus
1.13.11.63 for expression in Escherichia coli cells, genetically engineered to produce beta-carotene Drosophila melanogaster
1.13.11.63 into the vectors pCRII TOPO and pBAD-TOPO for expression in Escherichia coli XL1-blue cells Galleria mellonella
1.13.11.65 expression in Escherichia coli Drosophila melanogaster
1.13.11.65 expression in Escherichia coli Galleria mellonella

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.63 0.0104
-
 beta-carotene
-
 Galleria mellonella
1.13.11.63 0.02213
-
 alpha-carotene
-
 Galleria mellonella
1.13.11.65 0.0104
-
 beta,beta-carotene pH 6.8, 20°C Galleria mellonella
1.13.11.65 0.0221
-
 alpha-carotene pH 6.8, 20°C Galleria mellonella

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.63 57800
-
 theoretical Galleria mellonella
1.13.11.63 64000
-
 determined by SDS-PAGE and Western Blot analysis Galleria mellonella

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.63 Drosophila melanogaster Q9VFS2
-
-
1.13.11.63 Galleria mellonella
-
-
-
1.13.11.63 Mus musculus
-
-
-
1.13.11.65 Drosophila melanogaster Q9VFS2
-
-
1.13.11.65 Galleria mellonella A8Y9I2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.63 by affinity chromatography Galleria mellonella

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.63 head
-
 Galleria mellonella
-
1.13.11.63 pupa
-
 Galleria mellonella
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.63 alpha-carotene + ?
-
 Galleria mellonella 11-cis-retinal + all-trans-retinal + all-trans-alpha-retinal 32.7% 11-cis-retinal + 17.3% all-trans-retinal + 50.0% all-trans-alpha-retinal ?
1.13.11.63 beta-carotene + ?
-
 Galleria mellonella 11-cis-retinal + all-trans-retinal 41.2% 11-cis-retinal + 58.8% all-trans-retinal ?
1.13.11.63 beta-carotene + O2
-
 Drosophila melanogaster 11-cis-retinal + 13-cis-retinal + all-trans-retinal
-
 ?
1.13.11.63 beta-carotene + O2
-
 Mus musculus 13-cis-retinal + all-trans-retinal
-
 ?
1.13.11.63 cryptoxanthin + O2
-
 Galleria mellonella 11-cis-retinal + all-trans-retinal + 11-cis-3-hydroxy-retinal + all-trans-3-hydroxy-retinal 4.2% 11-cis-retinal + 45.8% all-trans-retinal + 35.1% 11-cis-3-hydroxy-retinal + 14.9% all-trans-3-hydroxy-retinal ?
1.13.11.63 lutein + O2
-
 Galleria mellonella 11-cis-3-hydroxy-retinal + all-trans-3-hydroxy-retinal + all-trans-3-hydroxy-alpha-retinal 39.9% 11-cis-3-hydroxy-retinal + 10.1% all-trans-3-hydroxy-retinal + 47.7% all-trans-3-hydroxy-alpha-retinal ?
1.13.11.63 zeaxanthin + O2
-
 Galleria mellonella (3R)-11-cis-3-hydroxyretinal + (3R)-all-trans-3-hydroxyretinal 41.5% 11-cis-3-hydroxy-retinal + 58.5% all-trans-3-hydroxy-retinal ?
1.13.11.65 alpha-carotene + O2
-
 Galleria mellonella ?
-
 ?
1.13.11.65 beta,beta-carotene + O2
-
 Drosophila melanogaster all-trans-retinal + 11-cis-retinal
-
 ?
1.13.11.65 beta,beta-carotene + O2
-
 Galleria mellonella all-trans-retinal + 11-cis-retinal enzyme catalyzes the production of 11-cis-retinal and all-trans-retinal, i.e. cleavage at the 15,15' double bond. The 11-cis-retinal product maybe thermally converted either into the all-trans or the 13-cis stereoisomer in the aqueous test solution ?
1.13.11.65 cryptoxanthin + O2
-
 Galleria mellonella 11-cis-3-hydroxyretinal + 11-cis-retinal 11-cis-3-hydroxyretinal and 11-cis-retinal exist in a molar ratio of 8:1, indicating that the enzyme preferentially isomerizes the half site of cryptoxanthin with the hydroxylated beta-ionone ring ?
1.13.11.65 lutein + O2
-
 Galleria mellonella ?
-
 ?
1.13.11.65 additional information enzyme combines isomerase and isomerooxygenase activity in a single polypeptide Drosophila melanogaster ?
-
 ?
1.13.11.65 additional information enzyme combines isomerase and isomerooxygenase activity in a single polypeptide Galleria mellonella ?
-
 ?
1.13.11.65 zeaxanthin + O2
-
 Galleria mellonella (3R)-all-trans-3-hydroxyretinal + (3R)-11-cis-3-hydroxyretinal
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.63 carotenoid oxygenase
-
 Mus musculus
1.13.11.63 carotenoid oxygenase
-
 Galleria mellonella
1.13.11.63 carotenoid oxygenase
-
 Drosophila melanogaster
1.13.11.63 CMO1
-
 Mus musculus
1.13.11.63 NinaB
-
 Mus musculus
1.13.11.63 NinaB
-
 Galleria mellonella
1.13.11.63 NinaB
-
 Drosophila melanogaster
1.13.11.65 NinaB
-
 Drosophila melanogaster
1.13.11.65 NinaB
-
 Galleria mellonella

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.63 28
-
 activity assay Galleria mellonella

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.63 0.00817
-
 alpha-carotene
-
 Galleria mellonella
1.13.11.63 0.00867
-
 beta-carotene
-
 Galleria mellonella
1.13.11.65 0.0082
-
 alpha-carotene pH 6.8, 20°C Galleria mellonella
1.13.11.65 0.0087
-
 beta,beta-carotene pH 6.8, 20°C Galleria mellonella

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.63 7.6
-
 activity assay Galleria mellonella