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Literature summary extracted from

  • de Sousa, S.M.; Rosselli, L.K.; Kiyota, E.; da Silva, J.C.; Souza, G.H.; Peroni, L.A.; Stach-Machado, D.R.; Eberlin, M.N.; Souza, A.P.; Koch, K.E.; Arruda, P.; Torriani, I.L.; Yunes, J.A.
    Structural and kinetic characterization of a maize aldose reductase (2009), Plant Physiol. Biochem., 47, 98-104.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.21 expressed as a recombinant His-tag fusion protein in Escherichia coli BL21(DE3) cells Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.21 4
-
 DL-glyceraldehyde in 50 mM sodium phosphate at pH 7.0 and 30°C Zea mays
1.1.1.21 108.1
-
 D-ribose in 50 mM sodium phosphate at pH 7.0 and 30°C Zea mays
1.1.1.21 179.5
-
 D-xylitol in 50 mM sodium phosphate at pH 7.0 and 30°C Zea mays
1.1.1.21 399.5
-
 D-sorbitol in 50 mM sodium phosphate at pH 7.0 and 30°C Zea mays
1.1.1.21 448.4
-
 D-arabinose in 50 mM sodium phosphate at pH 7.0 and 30°C Zea mays
1.1.1.21 466.8
-
 D-xylose in 50 mM sodium phosphate at pH 7.0 and 30°C Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.21 34560
-
 calculated from amino acid sequence Zea mays
1.1.1.21 34560
-
 recombinant enzyme, MALDI-TOF mass spectrometry Zea mays
1.1.1.21 34561
-
 1 * 34561, MALDI-TOF mass spectrometry Zea mays
1.1.1.21 35000
-
 SDS-PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.21 Zea mays A2T1W7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.21 agarose-IDA-Ni resin column chromatography, gel filtration Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.21 endosperm
-
 Zea mays
-
1.1.1.21 seed
-
 Zea mays
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.21 -20°C, the activity of the recombinant maize AKR4C7 enzyme is stable with no significant change in activity Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.21 D-arabinose + NADPH + H+
-
 Zea mays arabitol + NADP+
-
 ?
1.1.1.21 D-glucose + NADPH + H+ low affinity for D-glucose Zea mays D-sorbitol + NADP+
-
 ?
1.1.1.21 D-ribose + NADPH + H+
-
 Zea mays D-ribitol + NADP+
-
 ?
1.1.1.21 D-xylose + NADPH + H+
-
 Zea mays D-xylitol + NADP+
-
 ?
1.1.1.21 DL-glyceraldehyde + NADPH + H+
-
 Zea mays glycerol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.21 monomer 1 * 34561, MALDI-TOF mass spectrometry Zea mays

Synonyms

EC Number Synonyms Comment Organism
1.1.1.21 AKR4C7
-
 Zea mays
1.1.1.21 aldose reductase
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.21 NADPH
-
 Zea mays