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Literature summary extracted from

  • Detarsio, E.; Maurino, V.G.; Alvarez, C.E.; Mueller, G.L.; Andreo, C.S.; Drincovich, M.F.
    Maize cytosolic NADP-malic enzyme (ZmCytNADP-ME): a phylogenetically distant isoform specifically expressed in embryo and emerging roots (2008), Plant Mol. Biol., 68, 355-367.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.40 fumarate when assayed at malate concentrations of 0.2 mM, fumarate activates the enzyme by 80% over the control activity Zea mays
1.1.1.40 additional information after PEG 6000 treatment, the levels of cytosolic NADP-ME transcripts are enhanced by approximately 2.5times Zea mays
1.1.1.40 succinate when assayed at malate concentrations of 0.2 mM, succinate activates the enzyme by 121% over the control activity, at malate concentration of 1.2 mM, succinate activates the enzyme by 62% over the control activity Zea mays

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.40 expressed in Escherichia coli BL21 (DE3) cells Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.40 D-fructose-1,6-bisphosphate when assayed at malate concentrations of 0.2 mM, D-fructose-1,6-bisphosphate inhibits the enzyme by a 49% over the control activity Zea mays
1.1.1.40 additional information cytosolic NADP-ME expression in roots decreases with development, decreased levels of expression of cytosolic NADP-ME is observed in roots after incubating in solutions of Na2CO3 at pH 11.0 or NaHCO3 at pH 6.5; cytosolic NADP-ME is not inhibited by high malate concentrations at pH 7.0; NADP-ME is not affected by acetyl-CoA, CoA, pyruvate, L-alanine, alpha-ketoglutarate, glycerol-3-phosphate, 3-phospho-glycerate, and citrate Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.40 cytosol
-
 Zea mays 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.40 64000
-
 mature monomer of recombinant enzyme after enterokinase digestion, SDS-PAGE Zea mays
1.1.1.40 71600
-
 calculated from amino acid sequence Zea mays

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.40 Zea mays Q5G1U0 inbred line AX882 (Nidera)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.40 Ni-NTA column chromatography Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.40 embryo
-
 Zea mays
-
1.1.1.40 root emerging root Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.40 (S)-malate + NADP+
-
 Zea mays pyruvate + CO2 + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.40 hexamer or octamer
-
 Zea mays

Synonyms

EC Number Synonyms Comment Organism
1.1.1.40 NADP-malic enzyme
-
 Zea mays
1.1.1.40 NADP-ME
-
 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.40 7
-
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.40 NADP+
-
 Zea mays