EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.1.50 | pharmacology | Catharanthus roseus is the sole commercial source for monoterpenoid indole alkaloids vindoline and catharanthine, components of the anticancer dimers vinblastine and vincristine | Catharanthus roseus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.50 | PCR-amplification of cDNA, insertion into expression vector pET 30b, ligated into pGEM-TEasy, heat shock transformation of Escherichia coli DE3-MRF | Catharanthus roseus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.50 | loganin | reaction product | Catharanthus roseus | |
2.1.1.50 | S-adenosyl-L-homocysteine | - |
Catharanthus roseus | |
2.1.1.50 | S-adenosyl-L-methionine | - |
Catharanthus roseus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.50 | 14.76 | - |
loganic acid | pH 7.5, 37°C | Catharanthus roseus | |
2.1.1.50 | 742.1 | - |
S-adenosyl-L-methionine | pH 7.5, 37°C | Catharanthus roseus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.50 | 42020 | - |
calculated from 371-amino acid open reading frame | Catharanthus roseus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.50 | loganic acid + S-adenosyl-L-methionine | Catharanthus roseus | - |
loganin + S-adenosyl-L-homocysteine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.50 | Catharanthus roseus | B2KPR3 | cultivar Little Delicata, Madagaskar periwinkle | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.50 | crude recombinant enzyme, for enzyme activity location study upper and lower epidermis of leaves is abraded with carborundum, leaves rubbed with cotton swabs and then dipped in Trizol at 4°C and agitated | Catharanthus roseus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.50 | epidermis | - |
Catharanthus roseus | - |
2.1.1.50 | flower | highest activity | Catharanthus roseus | - |
2.1.1.50 | leaf | 10fold higher enzyme activity in epidermis extracts than in whole leaves, 50% lower activity in aged L2, L3, and L4 leaves compared to young L1 leaves | Catharanthus roseus | - |
2.1.1.50 | root | 4-8times lower activity than in the other tested plant organs | Catharanthus roseus | - |
2.1.1.50 | silique | similar activity as in leaves | Catharanthus roseus | - |
2.1.1.50 | stem | second highest activity | Catharanthus roseus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.50 | 0.000000517 | - |
secologanic acid, buffer (100 mM Tris-HCl, pH 7.5, 14 mM beta-mercaptoethanol, 25 mM KCl), 37°C, 30 min assay | Catharanthus roseus |
2.1.1.50 | 0.00000517 | - |
loganic acid, buffer (100 mM Tris-HCl, pH 7.5, 14 mM beta-mercaptoethanol, 25 mM KCl), 37°C, 30 min assay | Catharanthus roseus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.50 | loganic acid + S-adenosyl-L-methionine | - |
Catharanthus roseus | loganin + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.50 | secologanic acid + S-adenosyl-L-methionine | 10% activity compared to loganic acid | Catharanthus roseus | secologanin + S-adenosyl-L-homocysteine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.50 | LAMT | - |
Catharanthus roseus |
2.1.1.50 | loganic acid O-methyltransferase | - |
Catharanthus roseus |
2.1.1.50 | rLAMT | recombinant LAMT | Catharanthus roseus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.50 | 0.327 | - |
loganic acid | pH 7.5, 37°C | Catharanthus roseus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.50 | 7.5 | - |
- |
Catharanthus roseus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.50 | additional information | Mg2+ or K+ are not required for enzyme activity nor to enhance it | Catharanthus roseus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.50 | 0.2 | - |
S-adenosyl-L-methionine | pH 7.5, 37°C | Catharanthus roseus | |
2.1.1.50 | 0.215 | - |
loganin | pH 7.5, 37°C | Catharanthus roseus | |
2.1.1.50 | 0.4 | - |
S-adenosyl-L-homocysteine | pH 7.5, 37°C | Catharanthus roseus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.50 | metabolism | enzyme in the monoterpenoid indole alkaloid biosynthesis pathway | Catharanthus roseus |