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Literature summary extracted from

  • Bang, W.Y.; Jeong, I.S.; Kim, D.W.; Im, C.H.; Ji, C.; Hwang, S.M.; Kim, S.W.; Son, Y.S.; Jeong, J.; Shiina, T.; Bahk, J.D.
    Role of Arabidopsis CHL27 protein for photosynthesis, chloroplast development and gene expression profiling (2008), Plant Cell Physiol., 49, 1350-1363.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.81 PCR-amplification of cDNA from isolated RNA, PCR-products of enzyme-GFP fusion constructs are cloned into vector pPENSOTG and introduced into Arabidopsis protoplasts by polyethylene glycol-mediated transformation, complementation constructs are produced by joining PCR fragments of the enzyme promoter with the enzyme-GFP cDNA, cloned into vector pBIB-HYG, introduced into Agrobacterium GV3101, transformed into homozygous mutant plants via the floral dipping method Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.81 additional information mutant plants are complemented with enzyme construct fused to GFP at C-terminus under native promoter or a constitutive 35S-promoter control, suppressing the mutant phenotype, N-terminal deletion of the first 39 amino acids leads to location in the cytosol instead of chloroplast membrane (crucial localization signal) Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.81 chloroplast membrane N-terminal 39 amino acids are essential for chloroplast localization of the enzyme Arabidopsis thaliana 31969
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.81 magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Arabidopsis thaliana
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protochlorophyllide a + NADP+ + H2O
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?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.81 Arabidopsis thaliana Q9M591 chl27-t knock-down mutant
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.81 leaf
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Arabidopsis thaliana
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.81 magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
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Arabidopsis thaliana protochlorophyllide a + NADP+ + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.81 aerobic Mg-protoporphyrin IX monomethyl ester cyclase
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Arabidopsis thaliana
1.14.13.81 CHL27
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Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.14.13.81 malfunction the Arabidopsis chl27-t knock-down mutant has a T-DNA insertion within the promoter region reducing mRNA levels dramatically, growth is slowed down and plants look pale due to chloroplast development defects (unstacking of thylakoid membranes), chlorophyll fluorescence analysis shows a low photosynthetic activity, numerous nuclear genes involved in pigment biosynthesis, photosynthesis (light-harvesting complex I and II, photo system I and II), for iron acquisition, carbon fixation, cold acclimation, and for electron carriers are repressed, stress and detoxifying related gene expression is affected most prominently, more than 600 genes are repressed, a similar number of genes are induced Arabidopsis thaliana
1.14.13.81 metabolism enzyme is involved in the protochlorophyllide a synthesis Arabidopsis thaliana